Identification of Novel Shrimp Allergens and Their Diagnostic Utility for Phenotype Stratification in Korean Adults
- 주제(키워드) Shrimp allergy , anaphylaxis , whiteleg shrimp , myosin heavy chain , hemocyanin L1
- 주제(DDC) 570
- 발행기관 아주대학교 일반대학원
- 지도교수 Young-Min Ye
- 발행년도 2026
- 학위수여년월 2026. 8
- 학위명 석사
- 학과 및 전공 일반대학원 의생명과학과
- 실제URI http://www.dcollection.net/handler/ajou/000000036607
- 본문언어 영어
- 저작권 아주대학교 논문은 저작권에 의해 보호받습니다.
목차
I. Introduction 1
II. Materials and Methods 3
A. Study subjects 3
B. Preparation of whiteleg shrimp extract 4
C. IgE Immunoblotting 4
D. Mass Spectrometry 4
E. Gene synthesis, Expression and Purification of Allergenic Components 5
F. Validation of Anaphylaxis-Associated Allergens by Immunoblotting 6
G. Measurement of serum-specific IgE 6
1. ImmunoCAP assay 6
2. Component-specific ELISA 7
H. Statistical analysis 7
III. Results 8
A. Clinical Characteristics of Patients with Shrimp Allergy and Healthy Controls 8
B. Identification and Validation of Anaphylaxis-Associated Shrimp Allergens 10
C. Epitope Mapping of Myosin Heavy Chain by Fragmentation Analysis 12
D. Clinical Validation of Shrimp-Specific IgE Responses by Component-Specific ELISA 14
E. Determination of Optimal Cutoff Values for Phenotype- and Symptom-Based Classification 16
F. Additional Detection of Shrimp-Allergic Patients Beyond Conventional Shrimp Extract-Based Testing 20
IV. Discussion 26
References 31
Appendix 34
국문요약 37
