Enhanced oral peptide drug delivery using self-assembled gelatin-fatty acid nanoparticles
- 주제(키워드) Fatty acid types , Gelatin-fatty acid conjugates , Self-assembled nanoparticles , Intestinal permeability , Oral peptide delivery
- 주제(DDC) 615.1
- 발행기관 아주대학교 일반대학원
- 지도교수 Beom-Jin Lee
- 발행년도 2026
- 학위수여년월 2026. 8
- 학위명 석사
- 학과 및 전공 일반대학원 약학과
- 실제URI http://www.dcollection.net/handler/ajou/000000036510
- 본문언어 영어
- 저작권 아주대학교 논문은 저작권에 의해 보호받습니다.
목차
1. Introduction 1
2. Materials and methods 6
2.1. Materials 6
2.2. Preparation of GFN-PAS 7
2.2.1. Synthesis of gelatin-fatty acid conjugates (GFC) 7
2.2.2. Preparation of PAS-loaded GFN 8
2.3. HPLC analysis 10
2.4. Physicochemical characterizations of GFC and GFN-PAS 10
2.4.1. Dynamic light scattering (DLS) 10
2.4.2. Encapsulation efficiency (EE) and Drug loading (DL) 10
2.4.3. Determination of critical micelle concentration (CMC) 11
2.4.4. OPA assay for degree of substitution 11
2.4.5. Structural analysis FT-IR 11
2.4.6. Differential scanning calorimetry (DSC) 11
2.4.7. Surface morphology analysis by FE-SEM 12
2.4.8. Internal morphology analysis by FE-TEM 12
2.5. Stability study of PAS 12
2.6. Solubility study of PAS 13
2.7. In vitro permeability study 14
2.7.1. Permeability study in different gastrointestinal media 14
2.8. In vitro release study 15
2.8.1. Drug release in different gastrointestinal media 15
3. Results and discussions 16
3.1. Physicochemical characterization of GFC and GFN-PAS 16
3.2. Stability study of PAS 23
3.3. Solubility study of PAS 24
3.4. In vitro permeability study 31
3.4.1. Permeability study in different gastrointestinal media 31
3.5. In vitro release study 36
3.5.1. Drug release in different gastrointestinal media 36
4. Conclusion 39
5. References 40
국문초록 43
