아주대학교

목차

1. Introduction 1
2. Materials and methods 4
2.1. Materials 4
2.2. Preparation of APN 5
2.2.1. Screening processing conditions of blank APN 5
2.2.2. Effect alginate grades on the physicochemical properties of blank APNs 6
2.2.3. Preparation of LEU-loaded APN by W/O/W double-emulsion method 7
2.2.4. Preparation of QTP-loaded APN by O/W single emulsion method 8
2.3. HPLC analysis 12
2.4. EE and LC of drug-loaded APNs 12
2.5. Physicochemical characterization of APN, LEU-APN, and QTP-APN 13
2.5.1. Dynamic light scattering measurement 13
2.5.2. Short-term APN stability after reconstitution 14
2.5.3. Field emission scanning electron microscopy 14
2.5.4. Field emission transmission electron microscopy 15
2.6. Calcium-induced aggregation of APN 15
2.7. In vitro release study 15
3. Results and Discussion 17
3.1. Processing optimization for the preparation of APNs 17
3.2. Effect of alginate grade on physicochemical properties of blank APNs 23
3.3. Effect of alginate grades on the physicochemical properties of drug-loaded APNs 33
3.4. Effect of alginate grades on the release profiles of drug-loaded APNs 38
3.5. Calcium-responsive aggregation and release modulation of drug-loaded APNs 45
4. Conclusion 54
5. References 56
국문초록 60