Beneficial effects of Ahiflower (Buglossoides arvensis) oil on hyperlipidemia and thrombosis
아히플라워(Buglossoides arvensis) 오일의 고지질혈증 및 혈전증 억제 효과
- 주제(키워드) Ahiflower oil , Buglossoides arvensis , hyperlipidemia , thrombosis
- 주제(DDC) 610
- 발행기관 아주대학교 일반대학원
- 지도교수 Seon-Yong Jeong
- 발행년도 2026
- 학위수여년월 2026. 2
- 학위명 박사
- 학과 및 전공 일반대학원 의학과
- 실제URI http://www.dcollection.net/handler/ajou/000000035985
- 본문언어 영어
- 저작권 아주대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Beneficial effects of Ahiflower (Buglossoides arvensis) oil on hyperlipidemia and thrombosis Hyperlipidemia is a primary risk factor for cardiovascular diseases, characterized by its close association with endothelial dysfunction and thrombosis. While fish oil is a conventional source of omega-3 fatty acids, increasing concerns regarding its sustainability and sensory acceptance have shifted interest toward plant-derived alternatives. Ahiflower oil, extracted from the seeds of Buglossoides arvensis, is rich in stearidonic acid (SDA), α-linolenic acid (ALA), and γ-linolenic acid (GLA), suggesting potential efficacy in vascular function and thrombotic risk reduction. In this study, the beneficial effects of Ahiflower oil on hyperlipidemia and thrombosis were investigated using both in vitro and in vivo models. In tumor necrosis factor-α (TNF-α)-stimulated human umbilical vein endothelial cells (HUVECs), the expression of adhesion molecules (ICAM-1 and VCAM-1), endothelial NO synthase (eNOS) expression, NO production, and thromboxane B₂ (TXB₂) levels were evaluated and compared with fish oil. The in vivo efficacy of Ahiflower oil was further validated in three distinct mouse models: a high-fat diet (HFD) model of chronic hyperlipidemia, a poloxamer-407-induced model of acute hyperlipidemia, and a carrageenan-induced model of inflammation-driven thrombosis. Ahiflower oil significantly suppressed TNFα-induced mRNA expression of ICAM- 1 and VCAM-1 and reduced TXB₂ production, while simultaneously upregulating eNOS expression and NO release; notably, these effects surpassed those observed with fish oil. In HFD-fed mice, Ahiflower oil improved aortic adhesion-related gene expression, prolonged bleeding time, ameliorated plasma lipid profiles, and attenuated elevated AST and ALT levels. In the poloxamer-407 model, Ahiflower oil rapidly suppressed endothelial activation, inhibited platelet aggregation, and improved acute hyperlipidemia along with liver enzyme abnormalities. Crucially, in the carrageenan-induced model, Ahiflower oil reduced endothelial activation and platelet aggregation without significant alterations in systemic lipid profiles, thereby demonstrating a lipid-independent anti-thrombotic action. These results suggest that Ahiflower oil is a promising plant-derived, omega-3-rich candidate for mitigating endothelial dysfunction and thrombotic risk under both hyperlipidemic and inflammatory conditions. _________________________________________________________________________ Keywords: Buglossoides arvensis, Ahiflower oil; Triglycerides; Platelet aggregation; Omega-3 fatty acids; Hyperlipidemia; Thrombosis; Endothelial dysfunction
more목차
I. INTRODUCTION 1
II. MATERIALS AND METHODS 6
A. Reagents and materials 6
B. HUVEC culture 6
C. TNFα-induced endothelial dysfunction and Ahiflower oil treatment 6
D. Measurement of NO production 7
E. Quantification of TXB₂ 7
F. RNA isolation and quantitative reverse transcription PCR (qRT-PCR) in HUVECs 8
G. Animals and housing conditions 8
H. Ahiflower oil preparation and administration 8
I. HFD-induced hyperlipidemia model 9
J. Tail-bleeding time in HFD-fed mice 9
K. P-407–induced acute hyperlipidemia model 10
L. Carrageenan-induced thrombosis model 10
M. Platelet aggregation assay 11
N. Plasma lipid profile and liver enzyme measurements 11
O. Aortic RNA isolation and qRT-PCR 11
P. Statistical analysis 12
III. RESULTS 13
A. Ahiflower oil reverses TNFα-induced endothelial dysfunction in HUVECs 13
B. Ahiflower oil shows greater endothelial benefits than that of fish oil 15
C. Ahiflower oil normalizes aortic gene expression in HFD-induced hyperlipidemic mice 17
D. Ahiflower oil prolongs bleeding time and reduces platelet plug formation in HFD mice 19
E. Ahiflower oil improves plasma lipid profile and liver function in HFD mice 21
F. Ahiflower oil attenuates aortic endothelial activation in the P-407 acute hyperlipidemia model 23
G. Ahiflower oil inhibits platelet aggregation in P-407–treated mice 25
H. Ahiflower oil improves plasma lipid and liver enzyme profiles in the P- 407 model 27
I. Ahiflower oil reduces endothelial activation and platelet aggregation in carrageenan-induced thrombosis 29
IV. DISCUSSION 31
V. CONCLUSION 38
REFERENCES 39
국문요약 46
