유기농 복합추출물의 피부장벽강화 및 여드름/피부진정 효과에 관한 연구
- 주제(키워드) 유기농 , 여드름 , 피부진정 , 피부장벽강화
- 주제(DDC) 615.58
- 발행기관 아주대학교 글로벌제약임상대학원
- 지도교수 윤태종
- 발행년도 2026
- 학위수여년월 2026. 2
- 학위명 석사
- 학과 및 전공 글로벌제약임상대학원 글로벌제약임상약학과
- 실제URI http://www.dcollection.net/handler/ajou/000000035764
- 본문언어 한국어
- 저작권 아주대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
A Study on the Skin-Barrier-Enhancing, Acne- Impoving, and moisturizing Effects of Organic Plant Extract Complex Graduate School of Ajou University Department of Pharmacy Chang Kee Jong With the increasing prevalence of skin sensitivity and environmental stressors, there is a growing demand for natural and organic cosmetic ingredients that offer both high functionality and safety. In this study, we developed a liposomal formulation containing a complex of five organic plant extracts—Camellia sinensis, Chamomilla recutita, Centella asiatica, Aloe barbadensis, and Lavandula angustifolia—and evaluated its moisturizing, anti-inflammatory, barrier- strengthening, and sebum-regulating effects through a series of in vitro and in vivo studies. In vitro, the liposomal complex significantly suppressed nitric oxide (NO) production by 31% in LPS-stimulated cells, enhanced hyaluronic acid synthesis by 62%, and markedly upregulated the expression of barrier-related proteins, including filaggrin (3.4-fold) and involucrin (2.3-fold), compared with untreated controls. In vivo, a tape-stripping model was used to induce transient skin barrier disruption. Thirty minutes after application, the treated sites exhibited a significantly higher transepidermal water loss (TEWL) recovery rate (71.1%, p<0.001) and improved erythema index (71.5 A.U., p<0.001) compared with untreated sites. Cumulative irritation testing demonstrated a mean irritation score of 0.40, classifying the formulation as “low irritancy.” Furthermore, a 4-week clinical study demonstrated a significant reduction in sebum excretion (41%, p<0.001), accompanied by decreases in non-inflammatory (23.3%) and inflammatory (25.8%) acne lesion counts (p<0.01 for both), with no adverse events reported throughout the study period. Taken together, these findings indicate that the Organic MicroMelt Complex Calming liposomal formulation effectively reinforces the skin barrier, enhances hydration, suppresses inflammatory responses, and regulates sebum secretion, while maintaining an excellent safety profile. This formulation shows strong potential as a next-generation functional ingredient for organic cosmetics, particularly for sensitive and acne-prone skin. Keywords: Organic plant extracts, Liposome, Skin barrier, Moisturization, Anti- inflammatory, Sebum regulation, Acne, Human application study
more목차
Ⅰ. 서론 (INTRODUCTION) 1
1. 연구의 배경 및 필요성 1
2. 녹차, 캐모마일, 병풀, 알로에, 라벤더의 생리활성 2
3. 유기농 식물추출물의 리포좀화를 통한 피부효능 강화 3
4. 연구 목적 4
Ⅱ. 재료 및 방법 (MATERIAL AND METHODS) 5
1. 유기농 식물추출물 제조, 리포좀 합성 및 특성 분석 5
2. In vitro 세포 효능 평가 7
2.1 세포 배양 7
2.2 세포 배양 및 세포 독성 7
2.3 항염 평가(NO 생성 억제능 평가) 7
2.4 항염 평가(IL-6 생성 억제능 평가) 8
2.5 피부 보습 활성 8
2.6 피부 장벽 강화 활성 9
3. In vivo 인체 적용 시험 13
3.1 피부 자극 첩보 시험 13
3.2 경피 수분 손실량 회복 및 홍반도 측정 13
3.3 면포(여드름) 유발 가능성 확인 13
Ⅲ. 결과 (RESULTS) 15
1. 유기농식물 복합추추물-리포좀 입자 특성 15
2. In vitro 세포 효능 평가 17
2.1 항염 18
2.2 보습 18
2.3 피부 장벽 강화 19
3. In vivo (인체 적용 시험) 21
3.1 피부 자극 평가(피부 첩보첩) 21
3.2 경피 수분 손실량 23
3.3 면포(여드름) 유발 가능성 평가 25
Ⅳ. 고찰 (DISCUSSION) 27
Ⅴ. 참고문헌 (REFERENCES) 29
Ⅵ. ABSTRACT 33
