아주대학교

목차

1. Introduction 1
2. Materials and Methods 4
2.1. Materials 4
2.2. Synthesis of HOC 4
2.3. Preparation of each MEL formulations 5
2.3.1. Optimization of formulation by salt concentration 5
2.3.2. Optimization of formulation by HA concentration 8
2.3.3. Preparation of neutralized MEL (NM), neutralized MEL-HA (NM-HA) and neutralized MEL-HON (NM-HON) 10
2.4. Encapsulation efficiency (EE) and drug loading capacity (DL) 12
2.5. Characterization of NM, NM-HA and NM-HON 12
2.5.1. Fourier transform-infrared spectroscopy (FT-IR) 12
2.5.2. Field emission-scanning electron microscope (FE-SEM) 12
2.5.3. Field emission-transmission electron microscope (FE-TEM) 13
2.5.4. Differential scanning calorimetry (DSC) 13
2.5.5. High power X-ray diffraction (HP-XRD) 13
2.5.6. Dynamic light scattering measurement (DLS) 14
2.6. High performance liquid chromatography (HPLC) analysis 14
2.7. Stability study 14
2.7.1. Stability of salt and HA ratio formulations 14
2.7.2. Stability of NM, NM-HA and NM-HON 15
2.7.3. Stability of solid state 15
2.8. In vitro permeability study 15
2.9. In vitro drug release study 16
2.10. Cell culture and maintenance 17
2.11. In vitro cytotoxicity study 17
3. Results and discussions 18
3.1. Synthesis of HOC 18
3.2. Optimization of MEL stability by salt concentration and HA ratio 20
3.2.1. Effect of salt concentration on MEL stability 20
3.2.2. Effect of HA concentration on MEL stability 22
3.3. Stability of optimized NM formulations 24
3.3.1. Stability of NM, NM-HA and NM-HON 24
3.3.2. Stability of solid state 26
3.4. Nanoparticle properties of HON and NM-HON 28
3.5. Physicochemical characterization of NM, NM-HA and NM-HON 32
3.5.1. FE-SEM 32
3.5.2. FT-IR analysis 34
3.5.3. DSC analysis 36
3.5.4. HP-XRD analysis 38
3.6. In vitro permeability study 40
3.7. In vitro drug release study 42
3.8. In vitro cytotoxicity study 45
4. Conclusions 48
5. References 50
국문초록 52