Development of GFP ligands and linker optimization of GFP Proteolysis targeting chimeras (GFPTAC) as a general tool for protein study
- 주제(키워드) proteolysis- targeting chimeras (PROTACs) , Ubiquitin–proteasome system , green fluorescent protein (GFP) , targeted protein degradation (TPD) , binding affinity , GFPtargeting chimeras (GFPTACs)
- 주제(DDC) 547
- 발행기관 아주대학교 일반대학원
- 지도교수 최준원
- 발행년도 2026
- 학위수여년월 2026. 2
- 학위명 석사
- 학과 및 전공 일반대학원 분자과학기술학과
- 실제URI http://www.dcollection.net/handler/ajou/000000035576
- 본문언어 영어
- 저작권 아주대학교 논문은 저작권에 의해 보호받습니다.
목차
1. Introduction 1
1.1. Proteolysis targeting chimeras (PROTACs) 1
1.2. Ubiquitin–proteasome system (UPS) 2
1.3. Advantages of PROTACs 4
1.4. Green fluorescent protein (GFP) 5
1.5. GFP-targeting chimeras (GFPTACs). 5
2. Results and Discussion 7
2.1. Design and development of GFP ligands 7
2.2. Synthesis of modified GFP ligands 8
2.3. Structure-activity relationship (SAR) study of modified GFP ligands 13
2.4. Synthesis and SAR study of pyrrolidine-containing GFP ligands 17
2.5. Synthesis of linkers and GFPTACs 20
2.6. Activity evaluation of GFPTACs employing various types of linkers 25
3. Conclusions 26
4. Experimental Section 27
4.1. General information 27
4.2. Synthetic procedure 28
5. References 83
6. 1H and 13C NMR spectra 85
