아주대학교

목차

1. Introduction １
2. Materials and methods 5
2.1. Materials 5
2.2. Preparation of LOND 6
2.2.1. Preparation of LOC, LON 6
2.2.2. Preparation of LOND 7
2.3. HPLC analysis 8
2.4. Physicochemical characterizations of LOND 9
2.4.1. Dynamic light scattering (DLS) 9
2.4.2. Encapsulation efficiency (EE) and Loading content (LC) 9
2.4.3. Circular dichroism (CD) spectra 10
2.4.4. Field emission scanning electron microscope (FE-SEM) 10
2.4.5. Field emission transmission electron microscope (FE-TEM) 11
2.5. Physical stability 11
2.6. In vitro permeability study 11
2.7. In vitro release study 12
2.8. In vitro Lymphatic uptake study 13
2.8.1. Stability of INTRALIPID® 13
2.8.2. Entrapment efficiency in INTRALIPID® 14
2.8.3. Lymphatic uptake in INTRALIPID® 14
2.9. Cell culture and maintenance 15
2.10. In vitro cytotoxicity study 15
2.11. In vitro cellular uptake study 16
3. Results and discussions 18
3.1. Physicochemical characterization of LOND 18
3.2. Physical stability of LOND 24
3.3. In vitro permeability study 26
3.4. In vitro release study 29
3.4.1. (-) Esterase release study 29
3.4.2. (+) Esterase release study 32
3.5. In vitro lymphatic uptake study 35
3.5.1. Stability of INTRALIPID® 35
3.5.2. Entrapment efficiency in INTRALIPID® 37
3.5.3. Lymphatic uptake in INTRALIPID® 39
3.6. In vitro cytotoxicity study 42
3.7. In vitro cellular uptake study 45
4. Conclusion 47
5. References 48
국문초록 52