Study on the novel role of MLKL inhibitor in modulating inflammasome activity
- 주제(키워드) NLRP3 inflammasome , Necrosulfonamide (NSA) , Immunogenic cell death (ICD) , Inhibitor
- 주제(DDC) 570
- 발행기관 아주대학교 일반대학원
- 지도교수 You-Sun Kim
- 발행년도 2025
- 학위수여년월 2025. 8
- 학위명 석사
- 학과 및 전공 일반대학원 의생명과학과
- 실제URI http://www.dcollection.net/handler/ajou/000000035111
- 본문언어 영어
- 저작권 아주대학교 논문은 저작권에 의해 보호받습니다.
목차
I. INTRODUCTION 1
II. MATERIALS AND METHODS 3
A. Cell culture 3
B. Antibodies and chemical reagents 3
C. Inflammasome stimulation 3
D. Reducing and non-reducing western blot analysis 4
E. ASC oligomerization assay 4
F. BN-PAGE analysis 4
G. Plasmid transfection 5
H. Biotin-NSA pull-down assay 5
I. Purification of recombinant NLRP3 5
J. ELISA 6
K. Cytotoxicity Assays 6
L. Immunofluorescence staining 6
M. Statistical analysis 6
III. RESULTS 8
A. NSA blocks NLRP3 inflammasome activation at the level of caspase-1 cleavage. 8
B. NSA suppresses NLRP3, but not NLRC4 or AIM2 inflammasome activation. 14
C. NSA inhibits NLRP3 inflammasome activation independent of MLKL. 24
D. NSA inhibits the oligomerization of NLRP3. 26
E. NSA directly interacts with NLRP3. 30
IV. DISCUSSION 34
V. REFERENCES 36
국문요약 39
