YAP is involved in early-stage DNA damage sensing mediated by Ku70 or MRE11
YAP이 Ku70 또는 MRE11에 의해 매개되는 초기 DNA 손상 인식 과정에 관여한다
- 주제(키워드) YAP , DNA damage response
- 주제(DDC) 570
- 발행기관 아주대학교 일반대학원
- 지도교수 Jung-Soon Mo
- 발행년도 2025
- 학위수여년월 2025. 8
- 학위명 석사
- 학과 및 전공 일반대학원 의생명과학과
- 실제URI http://www.dcollection.net/handler/ajou/000000035016
- 본문언어 영어
- 저작권 아주대학교 논문은 저작권에 의해 보호받습니다.
목차
Ⅰ. Introduction 1
ⅠⅠ. Materials and Methods 11
1. Cell culture 11
2. Plasmid DNA transfection 11
3. CRISPR/Cas9 system for generating knock out cell lines 11
4. TA cloning 12
5. Western blot 12
6. Co-immunoprecipitation (Co-IP) 13
7. GST-fusion protein purification 13
8. In vitro GST pull-down assay 14
9. Subcellular fractionation 14
10. Molecular docking model 15
11. Live-cell imaging with laser micro-irradiation 15
12. Immunocytochemistry 16
13. Lentivirus production and generation of stable knockdown cell lines 16
14. Gene knockdown using siRNA 17
15. DNA repair reporter assay 17
16. Clonogenic assay 17
17. Antibodies 18
18. Chemicals 18
ⅠⅠⅠ. Results 19
1. YAP directly interacts with Ku70 19
2. The WW2 domain of YAP is the key mediator of its interaction with Ku70 22
3. The interaction between YAP and Ku70 is enhanced upon DNA damage 25
4. YAP depletion attenuates DDR and DSB repair efficiency 28
5. YAP depletion induces genomic instability 36
ⅠⅤ. Discussion 39
Ⅴ. References 43
국문 초록 55
