Study on the Inhibitory effect of IP3R- derived peptides on amyloid fibril formation of pathogenic huntingtin protein
- 주제(키워드) Huntington’s disease , Amyloid fibril , IP3R1 , Coiled coil , Inhibit , Cytotoxicity
- 주제(DDC) 547
- 발행기관 아주대학교 일반대학원
- 지도교수 Min-Duk Seo
- 발행년도 2025
- 학위수여년월 2025. 2
- 학위명 석사
- 학과 및 전공 일반대학원 분자과학기술학과
- 실제URI http://www.dcollection.net/handler/ajou/000000034663
- 본문언어 영어
- 저작권 아주대학교 논문은 저작권에 의해 보호받습니다.
목차
1. Introduction 1
2. Materials and Methods 5
2.1 Construct engineering and expression 5
2.1.1 ICT42 and variants (M1 and M2) 5
2.1.2 HttEx1-46Q 6
2.2 Purification 6
2.2.1 ICT42 and variants (M1 and M2) 6
2.2.2 HttEx1-46Q 7
2.3 Circular dichroism (CD) spectroscopy 8
2.4 Thioflavin T (ThT) fluorescence assay 8
2.5 Transmission Electron Microscopy (TEM) 9
3. Results 10
3.1 Construct design 10
3.1.1 Construct optimization 10
3.1.2 ICT42 and variants (M1 and M2) 12
3.2 Overexpression and purification of ICT42 and variants 14
3.3 Circular dichroism (CD) spectroscopy 17
3.3 Thioflavin T (ThT) fluorescence assay 19
3.4 Transmission Electron Microscopy (TEM) 21
4. Conclusion 23
5. References 25
