The anti-inflammatory effects of Aiouea padiformis extract via NLRP3 inflammasome inhibition
- 주제(키워드) NLRP3 inflammasome , anti-inflammation , plant extracts , Aiouea padiformis , Lauraceae
- 주제(DDC) 570
- 발행기관 아주대학교 일반대학원
- 지도교수 Yong Hwan Park
- 발행년도 2025
- 학위수여년월 2025. 2
- 학위명 석사
- 학과 및 전공 일반대학원 의생명과학과
- 실제URI http://www.dcollection.net/handler/ajou/000000034587
- 본문언어 영어
- 저작권 아주대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Aiouea padiformis, a member of the Lauraceae family found in tropical regions, has not been well-studied for its anti-inflammatory effects, despite reports of similar properties in other Aiouea species. In this study, I investigated the anti-inflammatory potential of Aiouea padiformis extract (AP) and its underlying mechanisms, focusing on the NOD-, LRR-, and pyrin domain-containing protein 3 (NLRP3) inflammasome. AP treatment inhibits NLRP3 inflammasome-mediated interleukin-1 beta (IL-1β) secretion in J774A.1 and THP-1 cells without affecting cell viability. Moreover, AP did not alter upstream events associated with NLRP3 inflammasome activation, such as NF-κB signaling, potassium efflux, or intracellular reactive oxygen species (ROS) production. Interestingly, AP significantly reduced the ATPase activity of NLRP3, leading to the inhibition of ASC oligomerization and speck formation. These results suggest that AP holds promise as a natural anti-inflammatory agent targeting NLRP3 inflammasome activity.
more목차
I. Introduction 1
II. Materials and Methods 5
1. Reagents and antibodies 5
2. Plant sample collection 6
3. In vitro study 6
3.1 Cell culture and stimulation 6
3.2 In vitro cell viability assay 7
3.3 NF-κB luciferase activity reporter gene assay 7
3.4 Measurement of cellular ROS assay 8
3.5 Measurement of mitochondrial membrane potential 8
3.6 Immunoprecipitation 8
3.7 Measurement ATPase activity of NLRP3 9
3.8 ASC oligomerization and ASC speck staining 9
4. Statistical analysis 10
III. Results 11
1. Screening and Identification of Aiouea padiformis as a Potent NLRP3 Inflammasome Inhibitor 11
2. Cell Viability Assessment Confirming Non-Cytotoxicity of AP 14
3. Inhibition of NLRP3-Mediated IL-1β and IL-18 Secretion by AP 15
4. Selective Inhibition of NLRP3 Inflammasome Activation by AP, Independent of AIM2 and NLRC4 17
5. Suppression of NLRP3 Inflammasome by AP without Effect on NF-κB Signaling 19
6. Reduction of NLRP3 Activation Independent of K+ Efflux, ROS, and Mitochondrial Instability by AP 21
7. AP-mediated Inhibition of NLRP3 Inflammasome Assembly by Impeding ATPase Activity 24
8. Suppression of NLRP3 Inflammasome Assembly by AP, Confirmed by Reduced ASC Oligomerization and Speck Formation 26
IV. Discussion 29
V. References 32
국문 초록 39
