Isolation and Characterization of Poly(butylene adipate-co-terephthalate) Degrading Yeast B. adeninivorans
- 주제(키워드) PBAT , 생분해
- 주제(DDC) 547
- 발행기관 아주대학교 일반대학원
- 지도교수 이평천
- 발행년도 2025
- 학위수여년월 2025. 2
- 학위명 석사
- 학과 및 전공 일반대학원 분자과학기술학과
- 실제URI http://www.dcollection.net/handler/ajou/000000034568
- 본문언어 영어
- 저작권 아주대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Poly(butylene adipate-co-terephthalate) (PBAT), a bioplastic made with aliphatic and aromatic hydrocarbons. PBAT is widely used with blending material to enhance the properties of other bioplastics. There are a few studies reporting microorganisms that can degrade PBAT. In this study, we found five kinds of PBAT-degrading microorganisms from various environmental samples and PBAT enriched soil and seawater. We characterized Blastobotrys sp. MB 2020, a new yeast strain capable of degrading PBAT. This strain demonstrated degradative activity on PBAT- containing medium and effectively metabolized 1,4-butanediol, a monomer of PBAT. We do GC/MS analysis to identify PBAT degradation products. This analytical approach detected 1,4-butanediol, adipic acid, terephthalic acid, and dimeric intermediates. Moreover, genomic and proteomic investigations identified putative PBAT-degradable enzymes, including lipases, cutinases, and the cutinase production pathway. These findings offer novel insights into the enzymatic and metabolic changes that enable PBAT biodegradation.
more목차
1. Introduction 1
2. Materials and Methods 3
2.1 Chemicals 3
2.2 CO₂ analysis of PBAT degradation in soil and seawater 4
2.3 Preparation of PBAT containing plate for screening 5
2.4 Screening and identification of PBAT-degrading microorganisms 6
2.5 Monitoring of PBAT degradation under solid and liquid conditions 7
2.6 HPLC analysis to confirm PBAT monomer assimilation 8
2.7 Monitoring of PBAT degradation under liquid condition 9
2.8 Gas chromatography–mass spectrometry (GC–MS) analysis 10
2.9 Whole genome sequencing analysis 11
2.10 Extracellular Protein Identification 11
3.Results 15
3.1 Preparation and analysis of environmental samples for PBAT- degrading microorganisms screening 15
3.2 Screening to isolate PBAT-degrading microorganisms 18
3.3 Characterization of PBAT-Degrading Yeast Strain MB 2020(Blastobotrys adeninivorans) 21
3.4 Monitoring of PBAT degradation under solid and liquid conditions 23
3.5 HPLC analysis to confirm PBAT monomer assimilation 27
3.6 PBAT powder degradation in liquid culture by Blastobotrys sp. MB 2020 30
3.7 Whole-genome sequencing analysis 34
3.8 Identification of extracellular proteins involved in PBAT degradation 37
4. Discussion 39
5. References 41
ABSTRACT IN KOREAN 44
