Self-Assembled Hyaluronic Acid Nanoparticles as Modulators of CD4+ Helper T cell Differentiation
- 주제(키워드) HA-NPs , inflammation , macrophages , CD4+helper T cells , Th17 cells
- 주제(DDC) 547
- 발행기관 아주대학교 일반대학원
- 지도교수 Wook Kim
- 발행년도 2025
- 학위수여년월 2025. 2
- 학위명 석사
- 학과 및 전공 일반대학원 분자과학기술학과
- 실제URI http://www.dcollection.net/handler/ajou/000000034407
- 본문언어 영어
- 저작권 아주대학교 논문은 저작권에 의해 보호받습니다.
목차
Ⅰ. INTRODUCTION 1
Ⅱ. MATERIALS and METHODS 5
1. Materials 5
2. Mice 5
3. Synthesis and characterization of HA-LCA NPs (HALN) 6
4. Synthesis and characterization of HA-3-oxoLCA NPs (3-oxoHALN) 6
5. Synthesis and characterization of PEG-LCA NPs (PEG-LN) 7
6. Cell culture and treatment 7
7. Isolation and T cell differentiation of naïve CD4+ T Lymphocytes 8
8. Cell viability assay 9
9. Cell cytotoxicity assay 9
10. RNA extraction and Real-Time quantitative PCR analysis 10
11. Flow cytometry analysis 10
12. Statistical analysis 11
Ⅲ. RESULTS 13
1. The physicochemical Characterization of HA-NPs 13
2. Size-dependent effect of HA-NPs on M1 macrophages 16
3. HALN and 3-oxoHALN suppress the TLR4-mediated M1 macrophage polarization 19
4. HALN and 3-oxoHALN restrain CD4+ T cell activation in Jurkat T cell 23
5. HALN and 3-oxoHALN inhibit Th17 differentiation 26
6. Evaluation of CD44 and TLR2 signaling pathways in the inhibition of Th17 differentiation mediated by HA-NPs 35
Ⅳ. DISCUSSION 39
Ⅴ. REFERENCE 42
Ⅵ. ABSTRACT IN KOREAN (국문초록) 46
