Inhibitory effects of CXCR4-Specific SDF-1 peptide(DGKPVSLSYR) on melanogenesis in human melanocytes
- 주제(키워드) Melanocytes , CXCR4 , SDF-1 peptide , MITF , TYR
- 주제(DDC) 570
- 발행기관 아주대학교 일반대학원
- 지도교수 Hee Young Kang
- 발행년도 2025
- 학위수여년월 2025. 2
- 학위명 석사
- 학과 및 전공 일반대학원 의생명과학과
- 실제URI http://www.dcollection.net/handler/ajou/000000034385
- 본문언어 영어
- 저작권 아주대학교 논문은 저작권에 의해 보호받습니다.
목차
Ⅰ. INTRODUCTUON 1
II. MATERIALS AND METHODS 3
1. Cell culture 3
2. Immunocytochemistry 3
3. MTT assay 3
4. RNA extraction and quantitative real-time PCR 4
5. Melanin content and tyrosinase activity assay 4
6. Enzyme-linked immunosorbent assay 5
7. Western blotting 5
8. Statistical analysis 5
9. List of primer sequences 5
III. RESULTS 6
1. Confirmation of CXCR4 expression levels in human primary melanocytes 6
2. Synthesis region of the SDF-1 peptide 8
3. Effects of SDF-1 peptide on morphological changes and cell viability of human primary melanocytes 9
4. Effects of SDF-1 peptide on MITF and TYR mRNA expression in human primary melanocytes 11
5. Effects of SDF-1 peptide on tyrosinase activity and total melanin content in human primary melanocytes 15
6. Effects of SDF-1 peptide on cAMP activity and p-CREB/CREB ratio in human primary melanocytes 18
IV. DISCUSSION 22
Ⅴ. CONCLUSION 24
Ⅵ. REFERENCES 25
