Development of a Non-Spectroscopic Immunosensing Platform Using Quantifiable Microscale Retroreflectors
- 주제(키워드) Biosensors , Retroreflection , POCT
- 주제(DDC) 547
- 발행기관 아주대학교 일반대학원
- 지도교수 윤현철
- 발행년도 2025
- 학위수여년월 2025. 2
- 학위명 석사
- 학과 및 전공 일반대학원 분자과학기술학과
- 실제URI http://www.dcollection.net/handler/ajou/000000034297
- 본문언어 한국어
- 저작권 아주대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Biosensors are analytical devices designed to detect or quantify specific target molecules using biological components. For applications in sensitive disease diagnosis, contaminant analysis, and disease monitoring, biosensors must provide high sensitivity and precise quantification. However, most current biosensors rely on costly, lab-scale equipment that limits accessibility, presenting a major barrier to their use in point-of-care testing (POCT) environments. In this study, we introduce a non-spectroscopic immunosensing platform that utilizes retroreflective Janus particles (RJPs) as optical probes for sensitive and simple quantification of analytes. To validate this approach, we selected β-hCG as a model analyte. Upon premixing the sample with RJPs functionalized with specific antibodies and biotin-conjugated antibodies, a biotinylated RJP-analyte complex is formed. When this complex is introduced into the chip, it binds to streptavidin in the test area. This interaction generates a visible retroreflective signal—quantifiable by counting white dots in the image of the test area—without requiring costly light sources or optical filters. To further evaluate the system's potential for commercial application and versatility, D-dimer was tested as an additional analyte, and the chip fabrication process was automated with a robotic protein dispenser. These results demonstrate that this platform is well-suited for sensitive detection of a variety of analytes, highlighting its potential applicability in POCT environment.
more목차
1. Introduction 1
1.1 Biosensors for qualitative and quantitative analysis 1
1.2 Retroreflection-based non-spectroscopic biosensor 3
1.3 Model analytes for the developed biosensor 6
1.3.1 β-human chorionic gonadotropin (β-hCG) 6
1.3.2 D-dimer 6
1.4 Sensing strategy of the developed biosensor utilizing the retroreflective Janus particle 8
1.5 Aim of thesis 10
2. Materials and methods 11
2.1 Reagents and apparatus 11
2.2 Fabrication of the β-hCG sensing chip for the proof-of-concept testing 12
2.3 Applying the automated robotic protein dispenser for the fabrication of the sensing chip 15
2.4 Fabrication of the retroreflective signaling probe 18
2.5 Strategy for the retroreflective signal analysis 21
2.6 Fabrication of the D-dimer sensing chip 22
3. Results and discussion 25
3.1 Proof of concept testing utilizing the β-hCG sensing chip 25
3.2 Fabrication and performance evaluation of β-hCG sensing chip utilizing an automated protein dispenser 30
3.3 Evaluation of sensing chip performance using β-hCG-spiked serum samples 34
3.4 Evaluating the applicability of the developed sensing chip for detecting additional analytes 36
4. Conclusions 40
5. References 42
