Inhibition of Virulence Gene Expression and Biofilm Formation by Heme-Enriched Corynebacterium Extract in Salmonella enterica and Escherichia coli
- 주제(키워드) virulence , biofilm formation , heme-enriched Corynebacterium extract
- 주제(DDC) 547
- 발행기관 아주대학교 일반대학원
- 지도교수 Hyunjin Yoon
- 발행년도 2024
- 학위수여년월 2024. 8
- 학위명 석사
- 학과 및 전공 일반대학원 분자과학기술학과
- 실제URI http://www.dcollection.net/handler/ajou/000000034230
- 본문언어 영어
- 저작권 아주대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Beef, along with poultry, is among the most consumed meats in the United States. However, excessive consumption poses health risks, prompting exploration of alternatives like plant-based options. Heme, produced by Corynebacterium glutamicum, is a potential key additive mimicking beef's flavor. In order to extrapolate the potential of hSCP as a dietary supplement, the influence of hSCP on the growth and virulence of foodborne pathogens was investigated, using Salmonella enterica serovar Typhimurium (S. Tm) and E. coli O157:H7 (EHEC). Supplementation with hSCP increased the growth of both species but decreased their motility and biofilm formation ability, two primary virulence determinants implicated in colonization during infection. Transcriptome analysis revealed that the genes required for motility and biofilm formation were accordantly downregulated by hSCP in both strains. Besides, hSCP caused transcriptional decreases in genes associated with Salmonella pathogenicity islands in S. Tm and the locus of enterocyte effacement pathogenicity in EHEC. Collectively, these findings underscore the advantageous impact of dietary hSCP on controlling the virulence of foodborne pathogens such as S. Tm and EHEC. Key words: virulence, biofilm formation, heme-enriched Corynebacterium extract
more목차
1. Introduction 1
2. Materials and Methods 6
2.1 Bacterial strains and culture condition 6
2.2 Heme-enriched Corynebacterium extract and hemin treatment 7
2.3 RNA extraction 8
2.4 RNA sequencing (RNA-Seq) and analysis 9
2.5 Quantitative real-time PCR analysis (qRT-PCR) 9
2.6 Motility assay 14
2.7 Biofilm assay 14
2.8 Determining Lactobacillus gasseri cholesterol assimilation in MRS 15
2.9 Bile salt hydrolase (BSH) activity 16
3. Results 17
3.1 Influence of hSCP and hemin on the growth of S. Typhimurium 14028s, E. coli O157:H7 ATCC 35150 17
3.2 Comprehensive transcriptomic analysis of S. Tm and EHEC under hSCP 19
3.3 Functional categorization of genes with significant transcriptional alterations 23
3.4 Differential effects between hSCP and hemin on the transcription of virulence-associated gene 27
3.5 Validation of the transcriptomes data using qRT-PCR. 30
3.6 Attenuated biofilm formation by hSCP in S. Tm and EHEC 38
3.7 Impact of hSCP on beneficial bacterium Lactobacillus gasseri 40
3.8 hSCP increased the beneficial cholesterol assimilation ability in L. gasseri 44
4.Discussion 47
5. Reference 50
