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Influences of Seasons, Latitudes, and Host Plants on Metabolic Changes in Cuscuta campestris Yunck.

  • 유찬우 (YuChanwoo, 아주대학교 일반대학원)

초록/요약

To determine whether the metabolomic patterns of Cuscuta campestris vary with season and latitude, samples were collected from parasitized individuals on the same host plant. In order to include any potential metabolomic difference resulting from host plant, the host plants were restricted to two species: Humulus japonicus and Artemisia sp. Metabolite profiles of C. campestris were analyzed by different seasons and latitudes conditions using gas chromatography-mass spectrometry (GC-MS). Principal component analysis (PCA) was conducted to examine the metabolite patterns and the PCA score plots of entire samples showed genetic influences more than environmental influences: seasons and latitudes. When looking at the metabolite patterns of C. campestris across the entire sample set, low latitude samples appear to be relatively distinct from high and middle latitude samples. For samples with H. japonicus as the host plant, the metabolite pattern showed a clear distinction between the middle latitude group and the other groups. these patterns did not show the expected high-middle-low order. On the other hand, samples with Artemisia sp. as the host plant, they tended to be well distinction among season and latitude groups mainly when October samples were included. Multivariate analysis of variance (MANOVA) was used to interpret whether statistically significant differences between the groups. The whole plant-seed group was significant in all comparison. In the season groups using PCA scores, high significances were observed only when compared to October, while comparisons between other seasons were less significant or not significant. In the latitude groups, the significant differences between groups were varied with each comparison. This study provides clues to understand the changes in metabolites of C. campestris from an ecological perspective. The limitations of this study included the absence of Artemisia sp. related samples collected in the October season, the inability to analyze the difference between the host plant with and without parasitism, and the absence of environmental data.

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목차

Ⅰ. Introduction 1
Ⅱ. Material and Methods 3
1. Study sites and sample collection methods 3
2. Metabolites analysis 6
2.1 Sample Preparation 6
2.2 Metabolite extraction 7
2.3 GC-MS data analysis 8
3. Statistical analysis 9
Ⅲ. Results 11
1. Sample collection information 11
2. Metabolite patterns across the entire sample set 13
3. Variability in metabolite patterns by season and latitude 18
3.1 Analysis about C. campestris parasitized on H. japonicus 18
3.2 Analysis about C. campestris parasitized on Artemisia sp. 25
Ⅳ. Discussion 31
Ⅴ. References 33
Ⅵ. Appendix 37
Ⅶ. 국문요약 39

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