아주대학교

목차

1. Introduction 1
2. Materials and Methods 5
2.1 Materials 5
2.2 Nectin-4 expression in human cancer and normal tissue datasets 6
2.3 Mouse immunization 6
2.4 Mouse serum ELISA 6
2.5 Cell fusion 7
2.6 ELISA of hybridoma-cultured supernatants 9
2.7 Hybridoma sequencing 10
2.8 Hybridoma cloning 11
2.9 Transfection of the cloned vector into mammalian cells 12
2.10 Antibody purification 12
2.11 Western blot 13
3. Results 15
3.1 Analysis of mRNA data of nectin-4 in human cancers and normal tissues 15
3.2 The expression levels of nectin-4 in ovarian cancer cells 15
3.3 Preparation of hybridoma 16
3.4 Screening of hybridoma subclones producing anti-nectin-4 mAbs 16
3.5 The amino acid sequence analysis of variable domains 17
3.6 Cloning variable domains into a single mammalian expression vector 17
3.7 Expression and purification of recombinant anti-nectin-4 mAbs 18
4. Discussion 19
5. Conclusion 22
References 32
-국문요약- 36