Sedum kamtschaticum exerts hypnotic effects via adenosine A2A receptor in mice
- 주제(키워드) Sedum kamtschaticum , Myricitrin , Hypnotic effect , Adenosine A2A receptor , GABAergic neuron
- 발행기관 아주대학교 일반대학원
- 지도교수 정이숙
- 발행년도 2024
- 학위수여년월 2024. 8
- 학위명 석사
- 학과 및 전공 일반대학원 약학과
- 실제URI http://www.dcollection.net/handler/ajou/000000034171
- 본문언어 영어
- 저작권 아주대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Insomnia is a common sleep disorder with significant societal and economic impacts. Current pharmacotherapy often has unwanted side effects, necessitating the exploration for new therapeutic drugs. This research investigated the hypnotic effects and mechanisms of Sedum kamtschaticum 30% ethanol extract (ESK) and one of its active compounds myricitrin using pentobarbital-induced sleep experiments, immunohistochemistry (IHC), immunofluorescence (IF), receptor binding assay and ELISA assay in mice. Our results from the pentobarbital-induced sleep experiments, showed that ESK and myricitrin reduced sleep latency and prolonged total sleep time in a dose-dependent manner. The findings from c-Fos immunostaining indicated that ESK and myricitrin enhanced neural activity in sleep-promoting VLPO GABAergic. We also confirmed the level of GABA released from VLPO GABAergic neurons. ESK and myricitrin increased the release of GABA in the hypothalamus. All these effects were significantly inhibited by SCH. Additionally, ESK showed a concentration-dependent binding affinity to the adenosine A2A receptor (A2AR). In conclusion, ESK and myricitrin have hypnotic effects and the underlying mechanism may be related to the activation of A2AR.
more목차
Ⅰ. INTRODUCTION 1
A. Sleep 1
B. Sleep regulation 2
C. Insomnia 3
D. Sedum kamtschaticum 3
E. Myricitrin 4
F. Aims of study 4
Ⅱ. MATERIALS AND METHODS 5
A. Materials 5
B. Sedum kamtschaticum Extract Preparation 5
C. Animals 6
D. pentobarbital-induced sleep test 6
E. Immunohistochemistry (IHC) 7
F. Immunofluorescence (IF) 8
G. Radioligand receptor binding assay 8
H. ELISA analysis 9
I. Statistical analysis 9
Ⅲ. RESULTS 10
A. ESK exerts hypnotic effect in pentobarbital-induced sleep model 10
B. The hypnotic effect of ESK is related to A2AR 12
C. ESK modulates sleep/wake-regulating regions in the mouse brain 14
D. ESK has binding affinity for A2AR 16
E. ESK compound exerts hypnotic effect in pentobarbital-induced sleep model 18
F. The hypnotic effect of myricitrin is related to A2AR 20
G. Myricitrin modulates sleep/wake-regulating regions in the mouse brain 22
H. ESK and myricitrin increase GABA release 24
IV. DISCUSSION 26
V. CONCLUSION 29
REFERENCES 30
