Site-Selective Antibody-Drug Conjugation Chemistry based on Inverse-electron- demand Diels-Alder Reaction
- 주제(키워드) Site selective drug conjugation , ADC platform , click chemistry
- 주제(DDC) 547
- 발행기관 아주대학교 일반대학원
- 지도교수 김은하
- 발행년도 2024
- 학위수여년월 2024. 8
- 학위명 석사
- 학과 및 전공 일반대학원 분자과학기술학과
- 실제URI http://www.dcollection.net/handler/ajou/000000034131
- 본문언어 한국어
- 저작권 아주대학교 논문은 저작권에 의해 보호받습니다.
목차
1. Introduction 1
1.1 Antibody-drug conjugate (ADC) 1
1.2 Importance of site selective drug conjugation to antibody 2
1.3 Site selective conjugation technology using click-to-release chemistry 4
1.4 Aim of thesis 6
2. Results and Discussion 7
2.1 Synthesis of peptide TCO NHS ester linker 7
2.2 Antibody-peptide conjugation 11
2.3 Drug conjugation by 'click to release' 15
3. Conclusion 18
4. Experimental Section 19
4.1 General information 19
4.2 Synthetic procedure 20
4.3 Protocols to produce ADC 33
5. References 34
6. Analytical results 36
6.1 HPLC chromatograms of antibody-peptide conjugates 36
6.2 1H NMR and 13C NMR spectra 46
