Crocetin and crocin synthesis via pathway gene expression in the genome of Saccharomyces cerevisiae
- 주제(키워드) Crocetin , Crocin
- 주제(DDC) 547
- 발행기관 아주대학교 일반대학원
- 지도교수 이평천
- 발행년도 2024
- 학위수여년월 2024. 8
- 학위명 석사
- 학과 및 전공 일반대학원 분자과학기술학과
- 실제URI http://www.dcollection.net/handler/ajou/000000034037
- 본문언어 한국어
- 저작권 아주대학교 논문은 저작권에 의해 보호받습니다.
목차
1. Introduction 1
2. Materials and Methods. 5
2.1 Strains and culture conditions 5
2.2 Construction of plasmids for the expression of crocin biosynthetic pathway enzyme 6
2.3 Enhancement of precursors to strengthen the GGPP pool 7
2.4 Integration of CCD, ALDH genes into CEN-ZP02 strain 8
2.5 Integration of crocin pathway UGT genes into CEN-ZA strain 10
2.6 Transcriptional analysis 11
2.7 Batch fermentation 11
2.8 Extraction of carotenoids. 12
2.9 Analysis of HPLC and mass spectrometry (MS). 13
3.Results. 20
3.1 Enhancement of zeaxanthin production by modulating ERG 9 promoter and overexpressing ERG13 and IDI1 in S. cerevisiae. 20
3.2 Integration and expression of CCD2 in S. cerevisiae 22
3.3 Integration and expression of ALDHs in S. cerevisiae 24
3.4 Construction of crocin-1 and 2 biosynthetic pathway. 26
3.5 Construction of crocin-3 and 4 biosynthetic pathway. 30
3.6 Enhancement of crocin-4 production through increased UDP-glucose supply 33
3.7 Batch fermentation of crocin-producing strain 36
4. Discussion 38
5. References. 41
ABSTRACT IN KOREAN 45
