Optimization of GFP Ligand and Development of GFP–Proteolysis Targeting Chimera (GFP–PROTAC) to Study Protein Functions
- 주제(키워드) methodology for studying protein functions , ubiquitin–proteasome system (UPS) , proteolysis–targeting chimera (PROTAC) , green fluorescent protein (GFP) , GFP– PROTAC
- 주제(DDC) 547
- 발행기관 아주대학교 일반대학원
- 지도교수 최준원
- 발행년도 2024
- 학위수여년월 2024. 8
- 학위명 석사
- 학과 및 전공 일반대학원 분자과학기술학과
- 실제URI http://www.dcollection.net/handler/ajou/000000034034
- 본문언어 영어
- 저작권 아주대학교 논문은 저작권에 의해 보호받습니다.
목차
1. Introduction 1
1.1. Importance of protein function study and the utility of GFP 1
1.2. Methodologies for studying protein functions 2
1.3. Proteolysis–targeting chimera (PROTAC) 4
1.4. GFP–PROTAC 7
2. Results and Discussion 9
2.1. Molecule design 9
2.2. Synthesis of GFP ligands and PROTACs 10
2.3. Structure–activity relationship: Binding affinity test of GFP with ligands 22
2.4. Evaluation of GFP–PROTACs performance in Colon26–GFP stable cell 29
3. Conclusions 31
4. Experimental Section 32
4.1. General information 32
4.2. Synthetic procedure 33
4.3. Microscale Thermophoresis (MST) Assay 109
4.4. Western blot 109
5. References 111
6. 1H and 13C NMR spectra 113
