목차

I. Introduction 1
II. Material and Methods 5
1. Antibodies 5
2. Chemicals 5
3. Cell culture and transfection 5
4. CRISPR/Cas9 system 6
5. Retroviral infection and generation of stable cell lines 6
6. Cell lysis and Western blotting 7
7. MTT assay 7
8. Cell apoptosis assay 8
9. Immunoprecipitation (IP) 8
10. Immunocytochemistry 9
11. RNA isolation and qRT-PCR (quantitative real-time polymerase chain reaction) 9
12. Wound healing assay 10
13. Clonal growth assay 11
14. TA cloning 11
15. Tumor isolation from mouse 12
III. Results 13
1. Cytotoxic effect of IALT induces apoptosis in SNU478 cells 13
2. IALT regulates YAP activity via Hippo pathway 16
3. Absence of LATS1/2 counteracts the effect of IALT in regulating YAP 21
4. IALT inhibits Cell growth, Cell Migration and Tumorigenesis by controlling YAP activity 30
IV. Discussion 37
V. References 40
국문 초록 43