The anti-tumor function of aryl hydrocarbon receptor expressed on macrophages in colorectal cancer
- 주제(키워드) Aryl hydrocarbon receptor (AhR) , Macrophages , Colorectal cancer , AOM/DSS , Anti-tumor immunity
- 주제(DDC) 615.1
- 발행기관 아주대학교 일반대학원
- 지도교수 장선영
- 발행년도 2024
- 학위수여년월 2024. 2
- 학위명 석사
- 학과 및 전공 일반대학원 약학과
- 실제URI http://www.dcollection.net/handler/ajou/000000033601
- 본문언어 영어
- 저작권 아주대학교 논문은 저작권에 의해 보호받습니다.
목차
Ⅰ. INTRODUCTION 1
Ⅱ. MATERIALS AND METHODS 2
1. Mice 2
2. Isolation of colonic lamina propria mononuclear cells 2
3. Bone marrow-derived macrophages 3
4. Real time-PCR 3
5. Flow cytometry 4
6. Magnetic sorting of F4/80+ cells 4
7. AOM/DSS-induced colorectal cancer 4
8. DSS-induced colitis 5
9. Tissue mRNA 5
10. Histology 5
11. ELISA 6
12. TCGA data analysis 6
13. Statics 6
Ⅲ. RESULTS 11
A. Absence of AhR in macrophages is more susceptible to AOM/DSS-induced colorectal cancer. 11
B. AHR-deficient BMDMs are more inflammatory under LPS stimulation. 14
C. AHR in macrophages reduces inflammatory damage in colon tissue. 17
D. Absence of AhR in macrophages leads to greater infiltration of pro-inflammatory macrophages. 19
E. AhR contributes to tumor suppression by blocking the STAT3 signaling pathway. 21
F. AhR in macrophages may activate the TGF-β/SMAD axis. 24
G. AhR in macrophages may also affect the function or activity of colonic B cells. 25
H. In human colon cancer, macrophages and STAT3 are correlated and AhR activity is decreased. 27
Ⅳ. DISCUSSION 29
Ⅴ. CONCLUSION 32
REFERENCES 33
KOREAN ABSTRACT 35
