목차

1. Introduction . 1
2. Materials and Methods . 4
2.1. Materials . 4
2.2. Preparation of fatty acid-conjugated hemagglutinin and self-assembled nanoparticle . 4
2.2.1. Synthesis of fatty acid-conjugated hemagglutinin . 4
2.2.2. Preparation of self-assembled NPs . 5
2.3 Physicochemical characterization of fatty acid-conjugated Heg . 5
2.3.1. Fourier-transform-infrared (FT-IR) spectrometer . 5
2.3.2. Matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) . 6
2.4. Secondary structure of fatty acid-conjugated Heg . 6
2.5. Critical Aggregation Concentration (CAC) of fatty acid-conjugated Heg . 7
2.6. Physicochemical characterization of the self-assembled NPS . 7
2.6.1. Field emission scanning electron microscopy (FE-SEM) . 7
2.6.2. Field emission transmission electron microscope (FE-TEM) . 8
2.7. Thermal stability in liquid state of self-assembled fatty acid conjugated Heg . 8
2.7.1. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) . 8
2.7.2 Size exclusion chromatography HPLC (SEC HPLC) . 9
2.7.3 Bicinchoninic acid (BCA) assay . 9
2.8. Thermal stability of self-assembled fatty acid conjugated Heg in physiological solution 9
2.9. Thermal stability of solid state fatty acid conjugated Heg . 10
3. Results and discussions . 11
3.1. Identification and physicochemical characterizations of fatty acid-conjugated Heg . 11
3.2. Secondary structures of fatty acid-conjugated Heg . 15
3.3. CAC of fatty acid-conjugated Heg . 18
3.4. Physicochemical characterizations of the self-assembled NPs . 20
3.5. Thermal stability in the liquid state of self-assembled fatty acid conjugated Heg . 22
3.6. Thermal stability of self-assembled fatty acid-conjugated Heg in physiological conditions . 26
3.7. Thermal stability of fatty acid-conjugated Heg in the solid state . 28
4. Conclusions . 32
5. References . 33
국문초록 . 37