목차

1. Introduction 1
2. Materials and Methods 4
2.1. Materials 4
2.2. Synthesis of OA-HSA conjugates (AOCs) 4
2.3. Preparations of AONs and DOX-AONs 5
2.3.1. Self-assembled AONs using desolvation method 5
2.3.2. Encapsulation of DOX into AONs (DOX-AONs) 6
2.3.3. Encapsulation efficiency (EE) and drug loading content (LC) 7
2.4. Physicochemical characterizations of AOCs, AONs, and DOX-AONs 7
2.4.1. Fourier transform-infrared (FT-IR) spectroscopy 7
2.4.2. Matrix-Assisted Laser Desorption Ionization - Time of Flight Mass Spectrometry (MALDI-TOF MS) 8
2.4.3. Dynamic light scattering (DLS) measurement 8
2.4.4. Field emission scanning electron microscopy (FE-SEM) and field emission transmission electron microscopy (FE-TEM) analysis 9
2.5. Cell culture and maintenance 9
2.6. Establishment and calibration of microfluidic BLC system 10
2.6.1. Calibration of BLC system 10
2.6.2. Establishment of microfluidic BLC system 14
2.6.3. Cell culturing on microfluidic BLC 18
2.6.4. Cell viability assay 19
2.6.5. Real-time ROS generation detection by sensor 19
2.6.6. Cellular images using confocal laser scanning microscopy 20
2.6.7. Estimation of IC50 and LD50 21
3. Results and discussions 22
3.1. Characterization of AOCs 22
3.2. Physicochemical characteristics of blank AONs and DOX-AONs 26
3.3. Comparison of the effectiveness of DOX-AONs under dynamic and static conditions 31
3.4. Effect of shear stress on cellular uptake 40
4. Conclusions 42
5. References 43
국문초록 47