목차

INTRODUCTION 1
MATERIALS AND METHODS 3
A. Cell culture 3
B. In vitro models of senescent melanocytes 3
C. Senescence associated β-galactosidase (SA-β-Gal) staining 3
D. Heterochromatin foci 3
E. Real-time PCR analysis 4
F. Western blotting 6
G. Melanin content and DOPA oxidase activity of tyrosinase 6
H. Electron microscopic observations 6
I. Immunocytochemical and immunohistochemical analysis 7
J. Single-cell transcriptomic analysis 7
K. Gene set enrichment analysis (GSEA) 8
L. Glycolytic flux analysis 8
M. Glycolysis inhibition and autophagy modulation 8
N. Measurement of ATP levels in melanocytes 9
O. Statistical analysis 9
RESULTS 10
A. Chronic UVB-exposure induces melanocyte senescence 10
B. Senescent melanocytes exhibit increased melanin contents without any increase of melanogenesis 16
C. Melanosome transport process and autophagy function are impaired in senescent melanocytes 23
D. Autophagy modulation exerts diverse effects on cellular senescence states in UVSM 31
E. Single-cell transcriptomics reveal glycolytic metabolism dysfunction in UVSM 38
F. Regulation of glycolytic metabolism alleviates senescence in melanocytes 46
DISCUSSION 54
CONCLUSION 61
REFERENCES 62
국문요약 67