Imaging Phenotype of Breast Cancer and Adjacent Parenchyma: Correlation with mRNA Expression of Hormone Receptors, HER2 and TP53
- 주제(키워드) Breast cancer , mRNA expression , imaging phenotype
- 주제(DDC) 610
- 발행기관 아주대학교
- 지도교수 김태희
- 발행년도 2023
- 학위수여년월 2023. 8
- 학위명 석사
- 학과 및 전공 일반대학원 의학과
- 실제URI http://www.dcollection.net/handler/ajou/000000033080
- 본문언어 영어
- 저작권 아주대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Purpose: The purpose of our study was to characterize the imaging phenotype of breast cancer and adjacent breast parenchymal tissue according to the mRNA expression of ER, PR, AR, HER2 and TP53. Materials and methods: From June 2020 to January 2023, 83 women who were newly diagnosed with breast cancer at our hospital were included. We obtained tissues from both breast cancer and adjacent parenchyma for the evaluation of mRNA expression using a reverse transcriptase-polymerase chain reaction (qRT-PCR). All 83 breast cancer tissues were adequate for PCR analysis and 59 of 83 tissues from adjacent parenchyma were adequate. Two radiologists with 11 and 3 years of experience in breast imaging interpreted the images according to the BIRADS lexicon. Radiologists evaluated the mammographic breast composition, background parenchymal enhancement and tumor imaging phenotypes. We also evaluated T2 bright signal intensity within the tumor and peritumoral edema on T2-weighted image. Results: Of 59 patients whose normal parenchymal tissue was available for PCR analysis, 17 patients had fatty breast or scattered areas of fibroglandular density on mammography and 42 patients had heterogeneously or extremely dense breast. Normal parenchymal tissue showing heterogeneous or extremely dense breast on MG showed significantly higher PR mRNA expression compared to patients with non-dense breast (p=0.015). In terms of BPE on breast MRI, ER-α, PR, HER2 and TP53 mRNA expressions were significantly higher in patients with mild, moderate or marked BPE than patients with minimal BPE (p=0.016, p=0.0001, p=0.038, p=0.035, respectively). Regarding the bright signal intensity (SI) within the mass on T2-weighted image, breast cancer with internal T2 bright SI showed higher expressions of ER-α, ER-β, PR, AR and TP53 mRNA compared to cancer without T2 bright SI. 62 (78%) of 79 cancers showed peritumoral edema on T2WI and 17 (22%) didn’t show. Expressions of ER-α, PR and AR mRNA were significantly lower in breast cancer with peritumoral edema on T2WI. Breast cancer with non-parallel orientation showed higher TP53 mRNA expression compared to parallel orientation (p=0.006). Breast cancer with calcification showed higher AR and HER2 mRNA expression compared to cancer without calcification (p=0.022 and p=0.022, respectively).
more목차
I. Introduction 1
II. Materials and methods 2
A. Study Participants 2
B. MR Imaging and Image Analysis 2
C. Reverse Transcription of CDNA and Quantitative Real-Time Polymerase Chain Reaction 2
D. Statistical Analysis 3
III. Results 4
IV. Discussion 5
V. List of Tables 7
A. Table 1. Primer sequences used for quantitative real time-PCR 7
B. Table 2. Comparison of mRNA expression in breast cancer according to the imaging
phenotypes 8
C. Table 3. Comparison of mRNA expression in breast parenchyma according to the
mammographic breast density and background parenchymal enhancement on MRI 10
D. Table 4. Correlation analysis for mRNA expression between breast cancer and adjacent
parenchyma 11
VI. List of Figures 12
A. Figure 1. 12
B. Figure 2. 13
C. Figure 3. 13
D. Figure 4. 14
E. Figure 5. 15
VII. References 16
