The glucocorticoid receptor (GR)-dependent regulation of NRF2 protein stability as an emerging drug target for cancer therapy
암 치료를 위한 새로운 약물 표적으로서 NRF2 단백질 안정성의 글루코코르티코이드 수용체(GR) 의존성 조절
- 주제(키워드) Glucocorticoid , Glucocorticoid receptor , NRF2 , Clobetasol propionate , reactive oxygen species
- 주제(DDC) 651.1
- 발행기관 아주대학교
- 지도교수 장선영
- 발행년도 2023
- 학위수여년월 2023. 8
- 학위명 석사
- 학과 및 전공 일반대학원 바이오헬스규제과학과
- 실제URI http://www.dcollection.net/handler/ajou/000000033069
- 본문언어 영어
- 저작권 아주대학교 논문은 저작권에 의해 보호받습니다.
목차
Ⅰ. INTRODUCTION 1
Ⅱ. MATERLIALS & METHODS 3
A. Cell culture, reagents Plasmid cloning and Lentivirus production 3
B. Plasmid cloning and Lentivirus production 3
C. Luciferase assay 5
D. Western blot 6
E. CO-IP (co-immunoprecipitation) 6
F. Immunostaining 7
Ⅲ. RESULTS 8
A. GR and NRF2 interaction. 8
B. The possibility that the DNA binding domain is required for NRF2 inhibition by CP. 11
C. Interaction between CP and the cysteine residues within the ligand binding domain (LBD) of the glucocorticoid receptor (GR). 14
D. Identifying novel compounds to offset steroid side effects caused by CP. 17
Ⅳ. DISCUSSION AND CONCLUSION 20
Ⅴ. REFERENCE 23
국문요약 25
