Potential role of microglia in axonal regeneration following facial nerve injury
- 주제(키워드) Microglia , Facial nerve injuries , Nerve Regeneration , PLX5622
- 주제(DDC) 570
- 발행기관 아주대학교
- 지도교수 김병곤
- 발행년도 2023
- 학위수여년월 2023. 8
- 학위명 석사
- 학과 및 전공 일반대학원 의생명과학과
- 실제URI http://www.dcollection.net/handler/ajou/000000032866
- 본문언어 영어
- 저작권 아주대학교 논문은 저작권에 의해 보호받습니다.
목차
Ⅰ. INTRODUCTION 1
A. Mechanism of spontaneous axon regeneration in the PNS neurons 1
B. Neuron-nonneuronal cell interaction in axonal regeneration 2
C. Anatomical structure of the facial nerve 4
D. Characteristics of the facial nerve injury model 4
E. Potential role of microglia in CNS neuroprotection and regeneration 6
F. PLX5622 is depleted microglia in the brain 7
G. Aim of this study 8
Ⅱ. MATERIALS AND METHODS 10
1. Animals and surgical procedure 10
2. Primary culture of bone marrow-derived macrophages 11
3. Primary microglia culture 11
4. Behavior tests 12
5. Fluorogold injection 13
6. Tissue processing and immunohistochemistry 14
7. Quantification 15
8. Statistical analysis 15
Ⅲ. RESULTS 19
1. Microglial cells are significantly activated in the facial motor nucleus after facial crush nerve injury 19
2. PLX5622 feeding efficiently suppressed the microglia in the facial motor nucleus 23
3. Microglia suppression delayed facial nerve regeneration 30
Ⅳ. DISCUSSION 36
REFERENCES 38
국문요약 46
