Single-cell Hemoprotein (heme-SCP) Induces Compositional Alterations in the Human Gut Microbiota
- 주제(키워드) hemin , heme-SCP , microbiota , gut
- 주제(DDC) 547
- 발행기관 아주대학교
- 지도교수 윤현진
- 발행년도 2023
- 학위수여년월 2023. 2
- 학위명 석사
- 학과 및 전공 일반대학원 분자과학기술학과
- 실제URI http://www.dcollection.net/handler/ajou/000000032778
- 본문언어 영어
- 저작권 아주대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Iron is an essential molecule in all living organisms. A variety of bacteria utilize iron in diverse biochemical reactions, mainly in the respiratory chain system as a cofactor of several enzymes, thereby accelerating bacterial growth. An increasing amount of evidence suggests pivotal roles of gut microbiota in human health concerned with immune system and metabolism disorder such as obesity, diabetes, and inflammatory bowel disease. This study investigated whether iron could influence the structural composition of gut microbiota. Human fecal microbiota were cultivated in a modified medium broth supplemented with hemin or heme-SCP, bacterial hemoprotein, and subjected to 16S rRNA sequencing. In comparison with the fecal microbiota inoculum, the in vitro cultivation with iron, regardless of hemin or heme-SCP, reduced the overall microbial diversity with changes in bacterial abundance. Despite the limitation of in vitro model, bacterial response to iron supplements was clearly differentiated between bacterial species. Hemin or heme-SCP tended to increase the abundance of Bacteroidetes and Proteobacteria phyla, while moderately decreasing the proportion of Firmicutes. However, species compositions were markedly different between hemin and heme-SCP treatments, which was probably because heme-SCP was dried biomass of hemoprotein-rich bacterial cells. The influence of hemin and heme-SCP on gut microbiota was re-evaluated by culturing ten bacterial species, the representative commensal species of human intestine, in combination or individually. The influence of iron supplements was different, being beneficial or detrimental depending on bacterial species, and the growth alterations observed in individual tests often disappeared in the co-culture system, implicating the importance of interspecies interactions in microbial community. This study demonstrated that heme compounds, hemin or heme-SCP, shaped the gut microbiota and the pros and cons effects on bacterial growth could be varied depending on bacterial species and cultivation methods.
more목차
1. Introduction 1
2. Material and Methods 4
2.1 Ethical Aproval 4
2.2 Fecal Sample Culture Condition 5
2.3 Bacterial Strains and Culture Condition 7
2.4 Heme-SCP and Hemin Treatment 10
2.5 GenominDNA Extraction 11
2.6 qPCR 12
3. Results 15
3.1 Compositional alteration of gut microbiota by heme-SCP 15
3.2 Compositional alteration of gut microbiota by hemin 22
3.3 Influence of heme-SCP and hemin on ten bacterial species in a co-culture model 29
3.4 Influence of heme-SCP and hemin on ten bacterial species in pure culture models 33
3.5 Response of three food-borne pathogens to heme-SCP and hemin 41
4. Discussion 44
5. Reference 49
