목차

1. Introduction 1
2. Experimental 3
2.1. Materials 4
2.2. Proliferation process of human mesenchymal stem cells (hMSCs) 4
2.3.PKH-Labeling of human mesenchymal stem cells (hMSCs) 4
2.4. ICG-Labeling of human mesenchymal stem cells (hMSCs) 4
2.5. Preparation of SMP-4-loaded click-crosslinked HA (Cx-HA) scaffold 5
2.6. Preparation of near-infrared (NIR) fluorescence-labeled HA, HA-DBCO and HA-Azide 6
2.7. Rheological measurements 6
2.8. SEM analysis 7
2.9. Displacement of ethanol analysis 7
2.10. Nano-indenter analysis 7
2.11. In vitro cytotoxicity of peptides or Cx-HA scaffold 8
2.12. In vitro migration of hMSCs towards Cx-HA, SMP-4-loaded Cx-HA, and SDF-1-loaded Cx-HA 8
2.13. In vitro release profiles of SMP-4-loaded Cx-HA and HA scaffold 9
2.14. In vitro degradation profiles of SMP-4-loaded Cx-HA and HA scaffold 9
2.15. Animal experiment 9
2.16. In vivo degradation image and release profile of SMP-4-loaded Cx-HA and HA scaffold 10
2.17. In vivo migration of ICG-labeling MSCs toward the SMP-4-loaded Cx-HA and HA scaffold 10
2.18 In vivo wound healing assessment via wound size analysis 11
2.19. In vivo wound healing assessment via analysis 11
2.20. In vivo Immunofluorescence staining of migrated hMSCs in SMP-4-loaded Cx-HA, SDF-1-loaded Cx-HA, or Cx-HA scaffold at skin tissue (CD29, CD31, CD44) 11
2.21. In vivo RT-PCR 13
3. Results&Discussion 15
3.1. Physical property measurement of click-crosslinking HA (Cx-HA) scaffold 15
3.2. In vitro Wound healing assay to hMSCs in the presence of SDF-1 or SMP-4 16
3.3. In vitro cytotoxicity to hMSCs in the presence of SDF-1 or SMP-4 scaffold 17
3.4. In vitro hMSCs migration toward SMP-4 or SDF-1 loaded Cx-HA scaffold 18
3.5. In vitro release profile of SMP-4-d-F from SMP-4-d-F-loaded HA or Cx-HA scaffold and degradation of HA or Cx-HA scaffold 20
3.6. In vivo release profile of SMP-4-d-F from SMP-4-d-F-loaded HA or Cx-HA scaffold and degradation of HA or Cx-HA scaffold 21
3.7. In vivo ICG-labeled hMSCs migration toward SMP-4 or SDF-1 loaded Cx-HA scaffold 22
3.8. In vivo evaluation of wound healing effects through MSC migration 24
3.9. In vivo masson's trichrome-stained histological images of wound healing effects to MSC migration 26
3.10. In vivo migration analysis via the surface (CD29 and CD44) staining in injected hMSCs skin tissue 30
3.11. In vivo migration analysis via the surface (CD31) staining in injected hMSCs skin tissue 33
3.12. In vivo migration analysis via the surface (CD29 and CD44) staining in endogenous mMSCs skin tissue 35
3.13. In vivo migration analysis via the surface (CD31) staining in endogenous mMSCs skin tissue 38
3.14. In vivo CD29, CD31, CD44, and CXCR4 RT-PCR of mouse wounded skin tissue 39
4. Conclusion 51
REFERENCES 52