목차

1. Introduction 1
2. Experimental 7
2.1. Materials 7
2.2. hMSCs culture 7
2.3. Labeling of hMSCs 8
2.4. Preparation of Cc-HA 9
2.5. Preparation of Cc-HA-BmP 10
2.6. Preparation of near-infrared (NIR) fluorescence-labeled HA, HA-Tet and HA-TCO 11
2.7. Rheological measurements 12
2.8. Measurement of injection force for injectable HA hydrogel formulations 12
2.9. In vitro cytotoxicity of peptide and hydrogel scaffold 13
2.10. In vitro migration of hMSCs 14
2.11. Osteogenic differentiation of hMSCs in the presence of BMP-2 or BmP in vitro 16
2.12. Osteogenic differentiation of hMSCs in Cc-HA, Cc-HA(+BmP) and Cc-HA-BmP hydrogel in vitro 18
2.13. Animal Experiments 19
2.14. In vivo imaging of injectable formulations 19
2.15. In vivo migration of MSCs in the hydrogel scaffolds 20
2.16. In vivo release profiles of SP1-F from SP1-F-loaded hydrogels 20
2.17. In vivo osteogenic differentiation of MSCs in the hydrogel scaffolds 21
2.18. Immunofluorescence staining of migrated hMSCs in Cc-HA, SP1-Cc-HA, SP1-Cc-HA(+BmP) and SP1-Cc-HA-BmP hydrogel in vivo (CD31) 22
2.19. Statistical analysis 23
3. Results & Discussion 24
3.1. Characterization of injectable click-crosslinked HA hydrogels 24
3.2. In vivo persistence of HA and Cc-HA hydrogel 27
3.3. In vitro cytotoxicity of peptide and hydrogel scaffold 29
3.4. Migration of hMSCs in the presence of SP or SP1 in vitro 31
3.5. In vitro hMSCs migration toward SP or SP1-loaded Cc-HA hydrogels 31
3.6. Osteogenic differentiation of hMSCs in the presence of BMP-2 or BmP in vitro 35
3.7. Osteogenic differentiation of hMSCs in Cc-HA, Cc-HA(+BmP) and Cc-HA-BmP hydrogel in vitro 36
3.8. In vivo hMSCs migration toward SP1-loaded Cc-HA hydrogels 39
3.9. In vivo real-time images of SP1-F from hydrogels 41
3.10. Osteogenic differentiation of hMSCs in Cc-HA, Cc-HA(+BmP) and Cc-HA-BmP hydrogel with SP1 in vivo 43
4. Conclusion 52
REFERENCES 53