Investigation of the effects of culture conditions on cell growth and tetraacetylphytosphingosine production in Wickerhamomyces ciferrii mutant strain
- 주제(키워드) Wickerhamomyces ciferrii , tetraacetylphytosphingosine , medium optimization
- 주제(DDC) 547
- 발행기관 아주대학교
- 지도교수 이평천
- 발행년도 2023
- 학위수여년월 2023. 2
- 학위명 석사
- 학과 및 전공 일반대학원 분자과학기술학과
- 실제URI http://www.dcollection.net/handler/ajou/000000032604
- 본문언어 영어
- 저작권 아주대학교 논문은 저작권에 의해 보호받습니다.
목차
1. Introduction 1
2. Materials and Methods 5
2.1 Strains, and culture conditions 5
2.2 Extraction and analysis of TAPS 5
2.3 96 well plate culture for C/N ratio 6
2.4 Omission test: trace metals and vitamins 6
2.5 Flask fermentation procedures 7
2.6 Plasmid construction for the expression of TAPS pathway enzymes 7
2.7 Transformation of W. ciferrii mutant 736 and flask cultivation 8
2.8 Transcriptional analysis 8
2.9 Batch fermentation under different conditions 9
2.10 Comparative fermentation with complex and modified defined medium 9
2.11 Analytical methods 9
3. Results and Discussion 13
3.1 Optimization of the C/N ratio 13
3.2 Single omission cultivation: vitamins and trace metals 15
3.3 TAPS production by the wild-type and mutant 736 in different concentrations of ZnSO4 17
3.4 Effect of different culture conditions on TAPS production 20
3.5 Comparative fermentation with complex and modified defined medium 22
3.6 Enhanced TAPS production of recombinant mutant 736 24
4. Conclusion 26
5. References 27
