Self-assembled hyaluronan nanoparticles for the treatment of skin barrier dysfunction in psoriasis
- 주제(키워드) Hyaluronan , nanoparticle , psoriasis , skin barrier function , keratinocyte
- 주제(DDC) 547
- 발행기관 아주대학교
- 지도교수 김욱
- 발행년도 2023
- 학위수여년월 2023. 2
- 학위명 석사
- 학과 및 전공 일반대학원 분자과학기술학과
- 실제URI http://www.dcollection.net/handler/ajou/000000032511
- 본문언어 영어
- 저작권 아주대학교 논문은 저작권에 의해 보호받습니다.
목차
I. INTRODUCTION 1
II. MATERIALS and METHODS 4
1. Materials 4
2. Preparation and characterization of HALN 5
3. Preparation of cy5.5-HALN 6
4. Cell 6
5. Real time quantitative PCR 6
6. Cell viability test 7
7. Western blot 8
8. Mice 9
9. IMQ-induced psoriasis mice model 9
10. In vivo skin penetration studies 9
11. Ex vivo skin penetration studies 10
12. Immunohistochemistry staining 11
13. Immunofluorescence staining 12
14. Statistical analysis 12
III. RESULTS 15
1. Synthesis and characterization of HALN 15
2. Subcutaneously administered HALN alleviates IMQ-induced psoriasis 19
3. Efficiency of skin penetration according to size of HALN 24
4. Size-dependent therapeutic effect of HALN in IMQ-induced mice model of psoriasis 27
5. HALN restores the skin barrier function in IMQ-induced mice model of psoriasis 32
6. Effect of HALN in IL-17A-stimulated keratinocytes 37
IV. DISCUSSION 42
V. REFERENCE 45
VI. ABSTRACT IN KOREAN (국문초록) 49
