The effect of metabolic disturbances on mouse brain pathology depending on APOE genotype
- 주제(키워드) High-fat diet , APOE4 , Obesity , Alzheimer’s disease , Pathology
- 주제(DDC) 570
- 발행기관 아주대학교
- 지도교수 박선아
- 발행년도 2022
- 학위수여년월 2022. 8
- 학위명 석사
- 학과 및 전공 일반대학원 의생명과학과
- 실제URI http://www.dcollection.net/handler/ajou/000000032248
- 본문언어 영어
- 저작권 아주대학교 논문은 저작권에 의해 보호받습니다.
목차
Ⅰ. INTRODUCTION 1
Ⅱ. MATERIALS AND METHODS 6
A. Animals and Experimental Diet 6
B. Body weight and Glucose level measurement 6
C. Behavior test 8
D. Collection of samples 9
E. Brain extraction 10
F. Western blotting 12
G. Immunohistochemistry 14
H. Statistical analysis 14
Ⅲ. RESULTS 16
1. Obesity phenotypes induced in HFD mice 16
2. Behavioral changes depending on genotype and diet 20
3. Reduced levels of insulin response in the 4KI but not in 3KI and KO mice 25
4. No changes of ApoE expression and lipidation according to HFD feeding 29
5. Neuronal and synaptic marker changes depending on the genotype and diet 32
6. Glial cell activation depending on genotype and diet 38
7. Hyperphosphorylated tau in the 4KI mice hippocampus 50
8. The phagocytic activation by microglia 62
Ⅳ. Discussion 65
Ⅴ. References 68
국문요약 72
