아주대학교

목차

1. Introduction 1
1.1. The senescent cell 1
1.2. The Golgi apparatus during aging 4
1.3. Zinc deficiency in aging 7
1.4. Research objectives 10
2. Experimental Methods 11
2.1. Cell culture and materials 11
2.2. Animal experiments 11
2.3. Western blot 11
2.4. BODIPY staining for flow cytometry 12
2.5. Immunofluorescence staining of cells 12
2.6. Immunoprecipitation 13
2.7. Isolation of Golgi-microtubule complexes 13
2.8. Nuclear and perinuclear fractionation 14
2.9. Tissue sampling 14
2.10. Cytosolic and nuclear fraction 14
2.11. Plasmid transfection 15
2.12. Real time quantitative polymerase chain reaction (qPCR) 15
2.13. Statistical analysis 15
3. Results 16
3.1. Aging led to an increase in Golgi stress 16
3.2. Aging disturbed Golgi structure and stacking 18
3.3. Golgi stress disturbed Golgi-derived microtubule assembly 20
3.4. Golgi stress impaired Golgi MT-mediated protein nuclear translocation 22
3.5. Golgi stress reduced the expression of hepatic zinc importer ZIP14 24
3.6. Zinc deficiency accelerated Golgi stress by inhibiting Golgi stacking 26
3.7. Zinc deficiency led to microtubule dysfunction 28
3.8. Zinc deficiency disturbed microtubule-mediated cellular signaling and epigenetic regulation 30
4. Discussion 33
5. Conclusions and Future Perspectives 38
6. References 39
7. Supplemental Information 47