Cleavage of MLKL at brace region through activation of caspase 3
- 주제(키워드) MLKL , caspase3 , cleavage
- 주제(DDC) 570
- 발행기관 아주대학교
- 지도교수 김유선
- 발행년도 2022
- 학위수여년월 2022. 8
- 학위명 석사
- 학과 및 전공 일반대학원 의생명과학과
- 실제URI http://www.dcollection.net/handler/ajou/000000032201
- 본문언어 영어
- 저작권 아주대학교 논문은 저작권에 의해 보호받습니다.
목차
I. INTRODUCTION 1
II. MATERIALS AND METHODS 3
A. Antibodies and chemical reagents 3
B. Cell culture 3
C. Plasmid and Lentiviral Transduction 3
D. Immunoblot analysis 4
E. Immunofluorescence staining 4
F. Cell imaging 4
III. RESULTS 6
A. Cleavage of MLKL under apoptotic conditions 6
B. Activation of caspase3 by intrinsic and extrinsic pathway cleaved MLKL. 10
C. Human MLKLD140 is the target site for caspase-mediated cleavage, and this is located at the brace region of MLKL. 16
D. Caspase3 generated MLKL-N terminal fragments in human cells but not in murine cells. 20
E. MLKL 1-140 fragment translocates to the membrane and nucleus. 23
F. MLKL 1-140 fragment does not affect cell death. 27
IV. DISCUSSION 32
V. REFERENCES 34
