아주대학교

목차

I. INTRODUCTION 1
II. MATERIALS & METHODS 7
A. Animal study 7
B. Oral glucose tolerance test (OGTT) and insulin tolerance test (ITT) 7
C. Blood biochemical analysis 8
D.Histological analysis 8
E. Next-Generation Sequencing (NGS)-based RNA-sequencing (RNA-seq) and in silico pathway analysis 9
F. RNA isolation and quantitative reverse-transcription PCR (qRT-PCR) 9
G. Western blotting 11
H. Cell culture and adipogenesis induction 12
I. Primary cell culture and adipogenesis induction 12
J. ORO staining of cells 13
K. Lentiviral infection of shRNA 13
L. Statistical analysis 14
III. RESULTS 15
A. Alteration of Cotl1 expression levels during adipogenesis induction in 3T3-L1 preadipocytes 15
B. Effects of Cotl1 deficiency on adipocyte differentiation in 3T3-L1 preadipocytes 17
C. Alteration of Cotl1 expression levels during adipogenesis induction in adipose-derived stem cells (ADSCs) 19
D. Effects of Cotl1 loss on adipogenesis in ADSCs isolated from Cotl1 knockout (Cotl1-/-) mice 21
E. Alteration of Cotl1 expression levels in mouse adipose tissues during normal diet (ND) and high-fat diet (HFD) feedings 23
F. Effects of Cotl1 loss on obesity-related phenotypes in HFD-fed mice 25
G. Effects of Cotl1 loss on hepatic steatosis and adipocyte hypertrophy in HFD-fed mice 27
H. Effects of Cotl1 loss on glucose metabolism-related phenotypes in HFD-fed mice 29
I. Effects of Cotl1 loss on plasma liver function parameters and lipid profiles in HFD-fed mice 32
J. In silico pathway analysis of differentially expressed genes (DEGs) in adipose tissues of HFD-fed WT and Cotl1-/- mice 34
K. Evaluation of mRNA expression levels of the DEGs identified by in silico pathway analysis 36
IV. DISCUSSION 38
V. CONCLUSION 44
REFERENCES 45
국문요약 57