The mechanism of Alzheimer’s disease risk gene-mediated tau secretion
- 주제(키워드) Tau , ApoE , Tau secretion , autophagy-lysosomal pathway
- 주제(DDC) 570
- 발행기관 아주대학교
- 지도교수 박선아
- 발행년도 2022
- 학위수여년월 2022. 8
- 학위명 석사
- 학과 및 전공 일반대학원 의생명과학과
- 실제URI http://www.dcollection.net/handler/ajou/000000032124
- 본문언어 영어
- 저작권 아주대학교 논문은 저작권에 의해 보호받습니다.
목차
I. INTRODUCTION 1
II. MATERIALS AND METHODS 4
A. Antibodies and reagents 4
B. Cell culture 4
C. Lentivirus production 4
D. Conditioned medium preparation 5
E. Cell lysis, SDS-PAGE, and Western blotting 6
F. MTT assay 7
G. Lysotracker staining 8
H. Proteinase K treatment 8
I. mRNA isolation and Quantitative PCR 9
III. RESULTS 12
1. ApoE effectively increased the tau secretion in vitro. 12
2. Tau secretion in the extracellular space is not caused by cell death. 17
3. The secretion of tau is increased by treating autophagy and proteasome inhibitors. 19
4. The extracellular tau is increased by ApoE overexpression through the autophagy-lysosomal pathway. 23
5. Tau is accumulated in autolysosomes. 24
6. Tau is secreted in the non-membrane form. 28
IV. DISCUSSION 31
V. REFERENCE 33
