Role of TINAGL1 derived from cancer-associated fibroblast on progression of diffuse-type gastric cancers
- 주제(키워드) Diffuse-type gastric cancer , Tumor microenvironment , Cancer-associated fibroblasts , Tubulointerstitial nephritis antigen-like 1
- 주제(DDC) 570
- 발행기관 아주대학교
- 지도교수 허훈
- 발행년도 2022
- 학위수여년월 2022. 8
- 학위명 박사
- 학과 및 전공 일반대학원 의생명과학과
- 실제URI http://www.dcollection.net/handler/ajou/000000031981
- 본문언어 영어
- 저작권 아주대학교 논문은 저작권에 의해 보호받습니다.
목차
I. INTRODUCTION 1
II MATERIALS AND METHODS 4
1. Cell culture 4
2. Preparation of conditioned media (CM) 4
3. Co-culture and treatment 4
4. Transwell migration assay 5
5. Soft agar assay 5
6. Cell proliferation assay 6
7. Transcriptome analysis 6
8. Proteomic analysis 6
9. RNA isolation and reverse transcriptase PCR (RT-PCR) 7
10. Quantitative reverse transcriptase PCR (qRT-PCR) 7
11. Enzyme-linked immunosorbent assay (ELISA) 8
12. Western blotting 8
13. Immunoprecipitation (IP) 9
14. Immunohistochemistry (ICC) 10
15. Phospho explorer antibody array 10
16. Small interfering RNA (siRNA) 11
17. Cell immortalization 12
18. Short hairpin RNA (shRNA) 12
19. Dual RNA-in situ hybridization (RNA-ISH) 12
20. Immunohistochemistry (IHC) 13
21. Public data 13
22. Animal model study 13
23. Statistical analysis 14
III. RESULTS 15
1. Integrated analysis for transcriptome and proteomics reveals that TINAGL1 is the specific molecule produced by CAFs 15
2. CAF-secreted TINAGL1 can interact with the integrin and activate FAK signaling pathway in DGC cells 24
3. TINAGL1-high CAFs can more contribute to the aggressiveness of DGC cells compared with NFs 33
4. Inhibition of the TINAGL1/integrin/FAK axis can alleviate CAF-induced aggressiveness of DGC cells 44
5. Stromal TINAGL1 expression is significantly correlated with the oncologic outcome of human DGC 52
IV. DISCUSSION 63
V. REFERENCES 69
국문요약 76
