Localization and roles of UCHL1 in the trans-differentiation of auditory supporting cells into hair cells
- 주제(키워드) UCHL1 , Cochlea , Auditory hair cells , Supporting cells , Trans-differentiation , mTOR
- 주제(DDC) 570
- 발행기관 아주대학교
- 지도교수 정연훈
- 발행년도 2022
- 학위수여년월 2022. 8
- 학위명 석사
- 학과 및 전공 일반대학원 의생명과학과
- 실제URI http://www.dcollection.net/handler/ajou/000000031933
- 본문언어 영어
- 저작권 아주대학교 논문은 저작권에 의해 보호받습니다.
목차
I INTRODUCTION 1
II MATERIALS AND METHODS 3
1. Organ of Corti explants 3
2. Drug treatment 3
3. Quantitative polymerase chain reaction 5
4. Immunofluorescence 5
5. Western blot analysis 6
6. 5-ethynyl-2′-deoxyuridine incorporation assay 6
7. Hematoxylin and eosin staining and histological analysis 7
8. Immunohistochemistry 7
9. Statistical analyses 7
III RESULTS 8
1. Dynamic expression of UCHL1 in the developing cochlea 8
2. A subpopulation of UCHL1-expressing cells switches to HCs under trans-differentiation 11
3. The UCHL1 inhibitor LDN-57444 promotes the trans-differentiation of SCs into HCs 14
4. mTOR activation by a UCHL1 inhibitor promotes HC trans-differentiation 19
IV DISCUSSION 23
V CONCLUSION 26
REFERENCES 27
국문요약 30
