Light quality stabilization of ASYMMETRIC LEAVES 1 induces leaf hyponasty in Arabidopsis by promoting auxin transport
- 주제(키워드) Molecular biology , Plant science , Plant molecular biology , Plant development
- 주제(DDC) 570
- 발행기관 아주대학교
- 지도교수 박대찬
- 발행년도 2022
- 학위수여년월 2022. 2
- 학위명 석사
- 학과 및 전공 일반대학원 생명과학과
- 실제URI http://www.dcollection.net/handler/ajou/000000031835
- 본문언어 영어
- 저작권 아주대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Plants have evolved an ability to adjust their morphology to the challenge imposed by a wide range of fluctuating environments. Competition for light among nearby plants induces shade avoidance responses including the elongation of hypocotyl and petiole, leaf hyponasty, and changes in leaf shape. These developmental adaptations are closely related to changes in light quality, especially the red to far-red ratio, and auxin levels. Despite recent progress in understanding shade avoidance responses, molecular mechanisms that mediate light and auxin signaling in adult plants are not well known. Here this study presents that ASYMMETRIC LEAVES 1 (AS1) conveys the light quality information for leaf hyponasty through the promotion of auxin transport. The AS1 overexpression plant shows increased leaf elevation angles throughout the day compared to wild-type plants, and the increased leaf angles in the AS1 overexpression plants are caused by the upward bending of petioles. In addition, transcriptome analysis identifies that most of the differentially expressed genes (DEGs) in the two different types of AS1 overexpression plants such as AUX/IAAs, SUARs, PINs are related to the auxin responses. Furthermore, the AS1-induced leaf hyponasty is dramatically reduced by low concentration of the polar auxin transporter inhibitor NPA, indicating that the function of AS1 plays a crucial role in the local distribution of auxin levels in the leaf hyponastic response. In the AS1 overexpression plants, the amount of auxin accumulated relatively more in petioles than in leaf blades. Morevoer, light quality regulation of AS1 protein stability, which highly accumulates in the nucleus under far-red conditions, supports the notion that AS1 may mediate far-red induced the leaf hyponastic response. All together, these findings suggest that AS1 function contributes to phenotypic plasticity that facilitates plants to adapt to variable environmental challenges by linking light and auxin signaling.
more목차
Ⅰ. Introduction 1
Ⅱ. Material and Method 7
Ⅱ.1. Plant materials and Growth conditions 7
Ⅱ.2. RNA isolation and RNA-seq analysis 7
Ⅱ.3. Protein preparation and nuclei isolation 7
Ⅱ.4. Measurement of leaf angle distribution 8
Ⅱ.5. Quantification of Auxin levels 8
Ⅲ. Result 9
Ⅲ.1. AS1 overexpression induces hyponastic leaf movement in Arabidopsis 9
Ⅲ.2. Transcriptome analysis shows that AS1 mediates Auxin signaling and positively regulate auxin related genes 13
Ⅲ.3. AS1 induces leaf hyponasty through regulation of auxin transport 22
Ⅲ.4. Changes in AS1 protein stability in response to light quality is important for leaf hyponasty 29
Ⅳ. Discussion 32
Ⅴ. Reference 36
국문요약 43
