Differentially expressed circulating exosomal microRNAs as biomarkers for disease severity in psoriasis patients
- 주제(키워드) Biomarker , Disease Severity , microRNAs , Psoriasis
- 주제(DDC) 610
- 발행기관 아주대학교
- 지도교수 이은소
- 발행년도 2022
- 학위수여년월 2022. 2
- 학위명 석사
- 학과 및 전공 일반대학원 의학과
- 실제URI http://www.dcollection.net/handler/ajou/000000031776
- 본문언어 영어
- 저작권 아주대학교 논문은 저작권에 의해 보호받습니다.
목차
INTRODUCTION 1
MATERIAL AND METHODS 3
A. Subjects and study flow 3
B. Isolation of EVs, RNA extraction and next-generation sequencing 5
C. microRNA real-time qPCR 5
D. Ingenuity Pathway Analysis (IPA®) 6
E. Statistical analysis 6
F. Ethics statement 6
RESULTS 7
A. Subject characteristics 7
B. Five up-regulated and 10 down-regulated exosomal miRNAs were differentially expressed between the groups based on NGS 9
C. Validation of candidate miRNAs by RT-qPCR 12
D. Downstream analysis of psoriasis biologic pathways through IPA for significant miRNAs 21
DISCUSSION 22
CONCLUSION 25
REFERENCES 26
