Effect of brain slice conditioned media (SCM) on differentiation of monocytes into microglia.
- 주제(키워드) Slice conditioned media , Monocytes , Microglia , Tmem119
- 주제(DDC) 570
- 발행기관 아주대학교
- 지도교수 박상면, 조은혜
- 발행년도 2022
- 학위수여년월 2022. 2
- 학위명 석사
- 학과 및 전공 일반대학원 의생명과학과
- 실제URI http://www.dcollection.net/handler/ajou/000000031618
- 본문언어 영어
- 저작권 아주대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
It has been reported that monocytes entered the injured brain, and differentiate into microglia, resident brain macrophages. In this study, I found that slice culture conditioned media (SCM) induced expression of Tmem119, a microglia specific marker, in bone marrow-derived monocytes (BMDM). In addition, SCM also increased phagocytic activity of monocytes. However, Tmem119 expression was not changed in the presence of a TLR4 agonist (LPS), or culture conditioned media from astrocytes (ACM) and neurons (NCM) treated with or without insults such as glutamate and ATP. These results suggest that factors from several types of cells in damaged brain tissues may cooperate to induce differentiation of monocytes into microglia.
more목차
Ⅰ. INTRODUCTION 1
A. Microglia. 1
B. Monocytes. 1
C. Monocytes in the damaged brain. 2
D. Infiltrating monocytes differentiated into microglia . 2
E. Specific Aims. 3
Ⅱ. MATERIALS AND METHODS. 4
A. Animal. 4
B. Stereotaxic injection 4
C. Immunostaining. 4
D. Monocytes culture. 5
E. Slice culture, preparation of slice culture conditioned media. 5
F. Western blot. 6
G. Statistical analysis. 6
Ⅲ. RESULTS 8
A. CD45(+) monocytes expressed Tmem119 in ATP injected brain. . 8
B. LPS did not induce differentiation of monocytes into microglia. . 10
C. Brain slice culture-conditioned media (SCM) induced differentiation of monocytes into microglia. 12
D. Immunocytochemistry: Expression of Tmem119 in monocytes induced by SCM. 14
E. SCM enhanced phagocytic activity in monocytes. 15
F. None of ACM, NCM, ATP, glutamate, and Conditioned media from ATP- or glutamate-treated astrocytes and neuron induced differentiation of monocytes into microglia. 16
Ⅳ. DISCUSSION 17
REFERENCE 19
국문요약. 25
