아주대학교

목차

Ⅰ. INTRODUCTION 1
Ⅱ. MATERIALS AND METHODS 4
Subjects and study approval 4
Plasma sample preparation 4
Purification of PBMCs and preparation of the cell lysates 5
Antibody validation 5
Immunoblot analysis 5
Flow cytometry of lysosomal pH 6
Statistical analysis 6
Ⅲ. RESULTS 7
A. Analysis of ALP-associated protein levels in the plasma of PD patients 7
1. Demographic and clinical characteristics of subjects for plasma analysis 7
2. Preparation of patient plasma samples for SDS-PAGE 7
3. Screening of biomarker candidate proteins associated with the autophagy-lysosome system in plasma samples 7
4. BAG2 levels are decreased in the plasma of patients with PD 8
5. Cathepsin D levels are decreased in the plasma of patients with PD 14
6. Association of plasma BAG2 and cathepsin D levels with clinical characteristics 17
7. The BAG2 and cathepsin D levels of plasma as potential biomarkers of PD 17
B. Analysis of ALP-associated protein levels and cellular activities of PD patient PBMCs 22
1. Demographic and clinical characteristics of subjects for PBMC analysis 22
2. The intracellular protein levels of LAMP1 and OPTN are altered in the PBMCs of PD patients 22
3. Autophagic activity analysis in the PBMCs of control and PD patients 22
4. Association of PBMC LAMP1 levels and autophagic flux with cardiac MIBG uptake in PD patients 23
5. Correlation analysis among the ALP-associated markers in PBMCs 33
6. The intracellular protein levels of LAMP1, OPTN and ATG5 as potential biomarkers of PD 33
Ⅳ. DISCUSSION 39
Ⅴ. CONCLUSION 43
REFERENCE 44