목차

ⅠIntroduction 1
ⅡMaterials and methods 4
1. Mice 4
2. RNA extraction 4
a. Organoid 4
b. In vivo 5
3. Real Time-PCR 5
4. Endotoxin analysis 6
5. Immuno-fluorescence (IF) staining 7
6. Organoid size and budding measurements 7
7. Organoid culture 7
8. HIF stabilizer 8
9. Statics 8
Ⅲ Results 11
A. The role of HIF stabilization on the gut organoid 11
B. HIF Stabilization promotes cell growth in organoid 13
C. HIF unaffected gut barrier at steady-state small intestine 15
D. HIF unaffected gut barrier at steady-state gut permeability 17
E. The role of AhR on gut barrier 19
F. AhR deficiency promotes cell growth 21
G. AhR deficiency promotes IECs proliferation and induces an increase in gut permeability 23
H. AhR deficiency leads to an increase in gut permeability 25
I. The role of HIF in AHR deficiency on the gut barrier 27
J. HIF did not increase uncontrolled cell proliferation caused by AhR deficiency 29
K. HIF restore the increased gut permeability caused by AhR deficiency 31
L. HIF inhibits the increase gut permeability caused by AhR deficiency in vivo 33
Ⅳ Discussion 35
ⅤConclusion 37
Reference 38
국문초록 40