Self-assembled Epitope-Peptide Amphiphile Micelles Improving an Antibody-Mediated Immune Response
- 주제(키워드) 수족구병 , Enterovirus 71 , 에피토프 , 펩타이드 양친매성 , 백신 , 체액성 면역 반응
- 발행기관 아주대학교
- 지도교수 진효언
- 발행년도 2021
- 학위수여년월 2021. 2
- 학위명 석사
- 학과 및 전공 일반대학원 약학과
- 실제URI http://www.dcollection.net/handler/ajou/000000030699
- 본문언어 영어
- 저작권 아주대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Hand, foot and mouth disease (HFMD) is an infectious disease caused by CoxsakievirusA16 (CVA16) or Enterovirus 71 (EV71). Among these, In the case of infection caused by EV71, serious complication of the heart and nervous system may occur. However, a vaccine or drug that can prevent this HFMD is not yet available. So, in this paper, we studied a peptide amphiphile (PA) vaccine that prevents HFMD by EV71 infection. PA is conjugated peptides with fatty acids, which self-assembled into micelle structures. Epitope peptide sequence was selected from EV71 virus particle 3 (VP3) and conjugated with spacer-linker and fatty acids. The PA structure was imaged using atomic force microscope and transmission electron microscope. In order to evaluate immune responses, our VP3PA vaccine, VP3-peptide, PBS (control) were injected intraperitoneal injection (i.p) into balb/c mice with adjuvant. VP3-specific IgG, IgG1 and IgG2a in collected serum from immunized mice was assayed by ELISA and Western blot for humoral immunity. VP3PA that we designed might overcome the limitation of peptide vaccine to prevent HFMD caused by EV71 through various experimental results.
more목차
1. Introduction 1
2. Materials and Methods 5
2.1. Material 5
2.2. Methods 6
2.2.1. Target recombinant viral capsid proteins (rVP3) characterization 6
2.2.2. Preparation of VP3PA 7
2.2.3. Characterization of VP3PA by AFM and TEM 7
2.2.4. Immunization in mice 8
2.2.5. Enzyme-linked immunosorbent assay (ELISA) 10
2.2.6. Western blot 10
2.2.7. Statistical analysis 11
3. Results and Discussion 12
3.1 Recombinant virus protein 3 was expression and characterization 12
3.2 VP3PA preparation 14
3.3 Self-assembly and characterization of VP3PA 16
3.4 Enzyme-linked immunosorbent assay (ELISA) 18
3.5 VP3-specific IgG detection by Western blot 20
4. Conclusion 22
References 23
