N2/Ar plasma induces necroptosis and regulates anti inflammatory responses in HNC
- 주제(키워드) N2/Ar plasma , Necroptosis , Head and neck cancer
- 발행기관 아주대학교
- 지도교수 김철호
- 발행년도 2021
- 학위수여년월 2021. 2
- 학위명 석사
- 학과 및 전공 일반대학원 분자과학기술학과
- 실제URI http://www.dcollection.net/handler/ajou/000000030529
- 본문언어 영어
- 저작권 아주대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Inflammatory responses play an important role in cancer survival and progression. For this reason, cancer can be treated by targeting inflammatory responses. It has been known that plasma treatment has an anti-inflammatory and anticancer effect. However, the mechanism by which N2/Ar plasma (NTP) induces cancer cell death and inhibits inflammation is unknown. In this study, we determined how NTP suppresses the progression of head and neck cancer (HNC). We found that the levels of inflammation-related and mitochondrial antiviral signaling (MAVS) proteins were elevated in HNC patient samples and that treatment of HNC cells with N2/Ar plasma decreased the expression of MAVS. In addition, N2/Ar plasma treatment inhibited the inflammatory response and induced necroptosis. We also identified that among the components of N2/Ar plasma-treated solution, peroxynitrite is mainly involved in the induction of necroptosis and upregulation of the expression of phosphorylated mixed lineage kinase domain-like protein. These results suggest that N2/Ar plasma might be used to treat HNC through the induction of necroptosis and inhibition of inflammatory responses.
more목차
Ⅰ. Introduction 1
Ⅱ. Material and Method 3
A. Cells culture and reagents 3
B. N2/Ar plasma characteristics and treatment 5
C. Cell counting 5
D. Cell viability assay 6
E. 3D spheroid culture 6
F. TUNEL assay 6
G. Western blot analysis 7
H. Induction / Inhibition of necroptosis 8
I. Immunocytochemistry microscopy 8
J. RNA interference analysis 9
K. Statistical analysis 9
L. JC-1, MitoRed, MitoSOX staining 10
M. ELISA 10
Ⅲ. Results 11
A. N2/Ar plasma induces cell death in HNC cells 11
B. N2/Ar plasma treatment induces necroptosis in FaDu and SNU1041 cells 12
C. RIP3 and MLKL level affects the sensitivity to N2/Ar plasma in HNC cells 13
D. Peroxynitrite of N2/Ar plasma induces necroptosis in HNC cells 14
E. N2/Ar plasma induces mitochondrial stress in HNC cells 15
F. N2/Ar plasma inhibits inflammation by degradation of MAVS aggregates in HNC cells 16
Ⅳ. Discussion 38
Ⅴ. Reference 41
